BMRBj - BMREntryMaster

Found 1 Document (1.699 seconds)

BMRB: 17849

2LHH

Solution structure of Ca2+-bound yCaM

Authors

Ogura, K., Takahashi, K., Kobashigawa, Y., Yoshida, R., Itoh, H., Yazawa, M., Inagaki, F.

Assembly

yCaM 123

Entity

1. yCaM 123 (polymer, Thiol state: not present), 128 monomers, 14250.47 Da Detail

SSNLTEEQIA EFKEAFALFD KDNNGSISSS ELATVMRSLG LSPSEAEVND LMNEIDVDGN HQIEFSEFLA LMSRQLKSND SEQELLEAFK VFDKNGDGLI SAAELKHVLT SIGEKLTDAE LEHHHHHH

2. CA (non-polymer, Thiol state: not present), 40.078 × 3 Da

Total weight

14370.704 Da

Max. entity weight

14250.47 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.1 %, Completeness: 92.1 %, Completeness (bb): 93.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.1 % (1335 of 1450)	94.4 % (706 of 748)	88.6 % (499 of 563)	93.5 % (130 of 139)
Backbone	93.1 % (713 of 766)	94.6 % (247 of 261)	92.1 % (348 of 378)	92.9 % (118 of 127)
Sidechain	91.4 % (737 of 806)	94.3 % (459 of 487)	86.6 % (266 of 307)	100.0 % (12 of 12)
Aromatic	55.9 % (57 of 102)	70.6 % (36 of 51)	41.2 % (21 of 51)
Methyl	98.5 % (134 of 136)	100.0 % (68 of 68)	97.1 % (66 of 68)

1. yCaM 123

SSNLTEEQIA EFKEAFALFD KDNNGSISSS ELATVMRSLG LSPSEAEVND LMNEIDVDGN HQIEFSEFLA LMSRQLKSND SEQELLEAFK VFDKNGDGLI SAAELKHVLT SIGEKLTDAE LEHHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	yCaM_123	[U-99% 13C; U-99% 15N]	1 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	CaCl2	natural abundance	5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 6 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 6 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LHH, Strand ID: A Detail

Release date

2012-01-31

Citation

Solution structures of yeast Saccharomyces cerevisiae calmodulin in calcium- and target peptide-bound states reveal similarities and differences to vertebrate calmodulin
Ogura, K., Kumeta, H., Takahasi, K., Kobashigawa, Y., Yoshida, R., Itoh, H., Yazawa, M., Inagaki, F.
Genes. Cells. (2012), 17, 159-172, PubMed 22280008 , DOI 10.1111/j.1365-2443.2012.01580.x , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 17850 released on 2012-01-31
Title Solution structure of Ca2+/CNA1 peptide-bound yCaM

Related entities 1. yCaM 123, : 1 : 290 entities Detail

Interaction partners 1. yCaM 123, : 75 interactors Detail

More details for 75 interactors identified by 10 citations

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC