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BMRB: 17855

2LHJ

Backbone 1H, 13C, and 15N Chemical Shift Assignments for high mobility group protein-like protein NHP1 from Babesia bovis T2Bo. Seattle Structure Genomics Center for Infectious Disease (SSGCID)

Authors

Barnwal, R.P., Varani, G.

Assembly

NHP1

Entity

1. NHP1 (polymer, Thiol state: not present), 97 monomers, 11117.57 Da Detail

MAGASDRTGV RRPRKAKKDP NAPKRALSSY MFFAKEKRVE IIAENPEIAK DVAAIGKMIG AAWNALSDEE KKPYERMSDE DRVRYEREKA EYAQRKV

Formula weight

11117.57 Da

Source organism

Babesia bovis

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.9 %, Completeness: 82.1 %, Completeness (bb): 93.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.1 % (943 of 1148)	76.0 % (466 of 613)	88.4 % (387 of 438)	92.8 % (90 of 97)
Backbone	93.2 % (533 of 572)	91.2 % (176 of 193)	94.8 % (272 of 287)	92.4 % (85 of 92)
Sidechain	72.8 % (487 of 669)	67.6 % (284 of 420)	81.1 % (198 of 244)	100.0 % (5 of 5)
Aromatic	35.9 % (23 of 64)	68.8 % (22 of 32)	0.0 % (0 of 31)	100.0 % (1 of 1)
Methyl	98.8 % (79 of 80)	97.5 % (39 of 40)	100.0 % (40 of 40)

1. high mobility group protein-like protein NHP1

MAGASDRTGV RRPRKAKKDP NAPKRALSSY MFFAKEKRVE IIAENPEIAK DVAAIGKMIG AAWNALSDEE KKPYERMSDE DRVRYEREKA EYAQRKV

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	high mobility group protein-like protein NHP1	[U-98% 13C; U-98% 15N]	1.2 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.28 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LHJ, Strand ID: A Detail

Release date

2011-08-30

Citation

1H and 15N Assigned Chemical Shifts for high mobility group protein-like protein NHP1 from Babesia bovis
Barnwal, R., Varani, G.
Not known

Related entities 1. NHP1, : 1 : 1 : 125 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC