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BMRB: 17858

2LHN

RNA-binding zinc finger protein

Authors

Brockmann, C., Neuhaus, D., Stewart, M.

Assembly

NAB2 404-483

Entity

1. NAB2 404-483 (polymer, Thiol state: all other bound), 80 monomers, 9207.623 Da Detail

GPLGSEKSLE QCKFGTHCTN KRCKYRHARS HIMCREGANC TRIDCLFGHP INEDCRFGVN CKNIYCLFRH PPGRVLPEKK

2. ZN (non-polymer, Thiol state: not present), 65.409 × 3 Da

Total weight

9403.85 Da

Max. entity weight

9207.623 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.8 %, Completeness (bb): 83.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.8 % (828 of 943)	93.8 % (471 of 502)	76.9 % (277 of 360)	98.8 % (80 of 81)
Backbone	83.4 % (392 of 470)	96.9 % (157 of 162)	69.1 % (161 of 233)	98.7 % (74 of 75)
Sidechain	93.2 % (509 of 546)	92.4 % (314 of 340)	94.5 % (189 of 200)	100.0 % (6 of 6)
Aromatic	73.7 % (56 of 76)	73.7 % (28 of 38)	73.7 % (28 of 38)
Methyl	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (27 of 27)

1. NAB2 404-483

GPLGSEKSLE QCKFGTHCTN KRCKYRHARS HIMCREGANC TRIDCLFGHP INEDCRFGVN CKNIYCLFRH PPGRVLPEKK

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 290 K, pH 6.75

#	Name	Isotope labeling	Concentration
1	TRIS	[U-2H]	50 mM
2	arginine	natural abundance	50 mM
3	glutamic acid	natural abundance	50 mM
4	zinc chloride	natural abundance	10 uM
5	mercaptoethanol	[U-2H]	5 mM
6	sodium chloride	natural abundance	50 mM
7	H2O	natural abundance	95 %
8	D20	natural abundance	5 %
9	NAB2_404-483	15N,13C U	1.2 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 290 K, pH 6.75, Details aligned sample for RDC measurements

#	Name	Isotope labeling	Concentration
10	TRIS	[U-2H]	50 mM
11	arginine	natural abundance	50 mM
12	glutamic acid	natural abundance	50 mM
13	zinc chloride	natural abundance	10 uM
14	mercaptoethanol	[U-2H]	5 mM
15	sodium chloride	natural abundance	50 mM
16	tobacco mosaic virus	natural abundance	25 mg/mL
17	H2O	natural abundance	95 %
18	D20	natural abundance	5 %
19	NAB2_404-483	15N,13C U	1.2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: -0.2 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: -0.2 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LHN, Strand ID: A Detail

Release date

2012-06-04

Citation

Structural basis for polyadenosine-RNA binding by Nab2 Zn fingers and its function in mRNA nuclear export
Brockmann, C., Soucek, S., Kuhlmann, S.I., Mills-Lujan, K., Kelly, S.M., Yang, J., Iglesias, N., Stutz, F., Corbett, A.H., Neuhaus, D., Stewart, M.
Structure (2012), 20, 1007-1018, PubMed 22560733 , DOI 10.1016/j.str.2012.03.011 , Abstract

Related entities 1. NAB2 404-483, : 1 : 3 : 28 entities Detail

Interaction partners 1. NAB2 404-483, : 29 interactors Detail

More details for 29 interactors identified by 8 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC