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BMRB: 17867

2LHU

Structural Insight into the Unique Cardiac Myosin Binding Protein-C Motif: A Partially Folded Domain

Authors

Howarth, J.W., Rosevear, P.R., Ramisetti, S., Nolan, K., Sadayappan, S.

Assembly

Myosin Binding Protein-C Motif

Entity

1. Myosin Binding Protein-C Motif (polymer, Thiol state: not present), 124 monomers, 13966.41 Da Detail

MGSSHHHHHH SSGLVPRGSH MHEAIGSGDL DLRSAFRRTS LAGAGRRTSD SHEDAGTLDF SSLLKKRDSF RRDSKLEAPA EEDVWEILRQ APPSEYERIA FQHGVTDLRG MLKRLKGMKQ DEKK

Formula weight

13966.41 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.5 %, Completeness: 46.6 %, Completeness (bb): 84.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	46.6 % (656 of 1408)	32.9 % (244 of 741)	57.3 % (311 of 543)	81.5 % (101 of 124)
Backbone	84.9 % (625 of 736)	85.5 % (218 of 255)	84.8 % (306 of 361)	84.2 % (101 of 120)
Sidechain	16.3 % (128 of 785)	5.3 % (26 of 486)	34.6 % (102 of 295)	0.0 % (0 of 4)
Aromatic	0.0 % (0 of 100)	0.0 % (0 of 50)	0.0 % (0 of 49)	0.0 % (0 of 1)
Methyl	15.3 % (15 of 98)	8.2 % (4 of 49)	22.4 % (11 of 49)

1. Myosin Binding Protein-C Motif

MGSSHHHHHH SSGLVPRGSH MHEAIGSGDL DLRSAFRRTS LAGAGRRTSD SHEDAGTLDF SSLLKKRDSF RRDSKLEAPA EEDVWEILRQ APPSEYERIA FQHGVTDLRG MLKRLKGMKQ DEKK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	myosin binding protein-C (255-357)	[U-13C; U-15N]	1.0 mM
2	TRIS	[U-2H]	20.0 mM
3	sodium chloride	natural abundance	50.0 mM
4	TCEP	natural abundance	2.0 mM
5	EDTA	natural abundance	1.0 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LHU, Strand ID: A Detail

Release date

2012-01-17

Citation

Structural insight into unique cardiac myosin-binding protein-C motif: a partially folded domain
Howarth, J.W., Ramisetti, S., Nolan, K., Sadayappan, S., Rosevear, P.R.
J. Biol. Chem. (2012), 287, 8254-8262, PubMed 22235120 , DOI 10.1074/jbc.M111.309591 , Abstract

Related entities 1. Myosin Binding Protein-C Motif, : 1 : 12 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC