BMRB: 17903

NMR Solution Structure of Yeast Iso-1-cytochrome c Mutant P71H in oxidized states

Authors

Lan, W., Wang, Z., Yang, Z., Zhu, J., Ying, T., Jiang, X., Zhang, X., Wu, H., Liu, M., Tan, X., Cao, C., Huang, Z.

Assembly

Iso-1-cytochrome

Entity

1. Iso-1-cytochrome (polymer, Thiol state: all free), 108 monomers, 12031.53 Da Detail

TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNHAKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

2. HEC (non-polymer, Thiol state: not present), 618.503 Da

Total weight

12650.033 Da

Max. entity weight

12031.53 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 80.5 %, Completeness (bb): 91.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	80.5 % (534 of 663)	80.5 % (534 of 663)
Backbone	91.1 % (205 of 225)	91.1 % (205 of 225)
Sidechain	75.1 % (329 of 438)	75.1 % (329 of 438)
Aromatic	62.5 % (35 of 56)	62.5 % (35 of 56)
Methyl	97.9 % (46 of 47)	97.9 % (46 of 47)

1. Iso-1-cytochrome

TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNHAKYI PGTKMAFGGL KKEKDRNDLI TYLKKATE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	Iso-1-cytochrome	natural abundance		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
4	Iso-1-cytochrome	natural abundance		1 mM
5	D2O	natural abundance		100 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2LIR, Strand ID: A Detail

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Release date

2011-12-05

Citation

Conformational toggling of yeast iso-1-cytochrome C in the oxidized and reduced states
Lan, W., Wang, Z., Yang, Z., Zhu, J., Ying, T., Jiang, X., Zhang, X., Wu, H., Liu, M., Tan, X., Cao, C., Huang, Z.
PLoS One (2011), 6, e27219-e27219, PubMed 22087268 , DOI 10.1371/journal.pone.0027219 , Abstract

Related entities 1. Iso-1-cytochrome, : 1 : 1 : 66 : 169 entities Detail

Interaction partners 1. Iso-1-cytochrome, : 4 interactors Detail

Experiments performed 6 experiments Detail