PFBD: High-throughput Strategy of Backbone fold Determination for small well-folded proteins in less than a day
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 33.9 % (256 of 755) | 19.7 % (78 of 395) | 40.6 % (119 of 293) | 88.1 % (59 of 67) |
Backbone | 64.9 % (239 of 368) | 50.4 % (63 of 125) | 63.9 % (117 of 183) | 98.3 % (59 of 60) |
Sidechain | 4.5 % (20 of 446) | 5.6 % (15 of 270) | 3.0 % (5 of 169) | 0.0 % (0 of 7) |
Aromatic | 0.0 % (0 of 58) | 0.0 % (0 of 29) | 0.0 % (0 of 27) | 0.0 % (0 of 2) |
Methyl | 2.9 % (2 of 70) | 2.9 % (1 of 35) | 2.9 % (1 of 35) |
1. SH3
MDETGKELVL ALYDYQEKSP REVTMKKGDI LTLLNSTNKD WWKVEVNDRQ GFVPAAYVKK LDSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | SH3 | [U-99% 13C; U-99% 15N] | 1.2 mM | |
2 | sodium phosphate | natural abundance | 50 (±0.05) mM | |
3 | NaCl | natural abundance | 150 (±0.05) mM | |
4 | H2O | natural abundance | 90 (±0.05) % | |
5 | D2O | natural abundance | 10 (±0.05) % |