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BMRB: 17934

2M38

AIDA1 PTB domain

Authors

Donaldson, L.

Assembly

AIDA1 PTB domain

Entity

1. AIDA1 PTB domain (polymer, Thiol state: all free), 153 monomers, 17042.35 Da Detail

STPVQAWQHH PEKLIAQSCD YKAAYLGSML IKELRGTEST QDACAKMRAN CQKSTEQMKK VPTIILSVSA KGVKFIDATN KNIIAEHEIR NISCAAQDPE DLSTFAYITK DLKSNHHYCH VFTAFDVNLA AEIILTLGQA FEVAYQLALQ ARK

Formula weight

17042.35 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.4 %, Completeness: 80.2 %, Completeness (bb): 88.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.2 % (1426 of 1778)	77.8 % (715 of 919)	82.3 % (570 of 693)	84.9 % (141 of 166)
Backbone	88.9 % (809 of 910)	88.6 % (271 of 306)	88.8 % (404 of 455)	89.9 % (134 of 149)
Sidechain	74.0 % (753 of 1017)	72.4 % (444 of 613)	78.0 % (302 of 387)	41.2 % (7 of 17)
Aromatic	42.9 % (54 of 126)	44.4 % (28 of 63)	40.3 % (25 of 62)	100.0 % (1 of 1)
Methyl	93.5 % (172 of 184)	94.6 % (87 of 92)	92.4 % (85 of 92)

1. AIDA1 PTB domain

STPVQAWQHH PEKLIAQSCD YKAAYLGSML IKELRGTEST QDACAKMRAN CQKSTEQMKK VPTIILSVSA KGVKFIDATN KNIIAEHEIR NISCAAQDPE DLSTFAYITK DLKSNHHYCH VFTAFDVNLA AEIILTLGQA FEVAYQLALQ ARK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	AIDA1 PTB domain	natural abundance	0.8 mM
2	sodium azide	natural abundance	0.05 %
3	sodium chloride	natural abundance	150 mM
4	sodium phosphate	natural abundance	20 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M38, Strand ID: A Detail

Release date

2012-09-16

Citation

Solution structure and peptide binding of the PTB domain from the AIDA1 postsynaptic signaling scaffolding protein
Smirnova, E., Shanbhag, R., Kurabi, A., Mobli, M., Kwan, J.J., Donaldson, L.W.
PLoS One (2013), 8, e65605-e65605, PubMed 23799029 , DOI 10.1371/journal.pone.0065605 , Abstract

Related entities 1. AIDA1 PTB domain, : 1 : 4 : 167 entities Detail

Interaction partners 1. AIDA1 PTB domain, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 10 experiments Detail

1 3D 1H-13C NOESY aliphatic