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BMRB: 17942

2M3M

1H, 13C and 15N resonance assignment of a complex constisting of hDlg/SAP-97 residues 318-406 and HPV type 51 E6 protein residues 141-151

Authors

Mischo, A., Ohlenschlaeger, O., Goerlach, M.

Assembly

Complex of hDlg and E6

Entity

1. hDlg (polymer, Thiol state: all free), 97 monomers, 10381.73 Da Detail

MEIKLIKGPK GLGFSIAGGV GNQHIPGDNS IYVTKIIEGG AAHKDGKLQI GDKLLAVNNV CLEEVTHEEA VTALKNTSDF VYLKVAKPTG SHHHHHH

2. E6 protein fragment of HPV type 51 (polymer, Thiol state: all free), 11 monomers, 1398.483 Da Detail

XRTRQRNETQ V

Total weight

11780.214 Da

Max. entity weight

10381.73 Da

Source organism

Homo sapiens , human papillomavirus 51

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 96.3 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.3 % (1173 of 1218)	96.4 % (608 of 631)	96.4 % (456 of 473)	95.6 % (109 of 114)
Backbone	97.3 % (619 of 636)	97.3 % (217 of 223)	98.1 % (303 of 309)	95.2 % (99 of 104)
Sidechain	95.6 % (647 of 677)	95.8 % (391 of 408)	95.0 % (246 of 259)	100.0 % (10 of 10)
Aromatic	72.2 % (52 of 72)	72.2 % (26 of 36)	72.2 % (26 of 36)
Methyl	100.0 % (128 of 128)	100.0 % (64 of 64)	100.0 % (64 of 64)

1. hDlg

MEIKLIKGPK GLGFSIAGGV GNQHIPGDNS IYVTKIIEGG AAHKDGKLQI GDKLLAVNNV CLEEVTHEEA VTALKNTSDF VYLKVAKPTG SHHHHHH

2. E6 protein fragment of HPV type 51

XRTRQRNETQ V

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	hDlg	[U-100% 13C; U-100% 15N]	0.8 mM
2	E6 protein fragment of HPV type 51	natural abundance	2 mM
3	sodium phosphate	natural abundance	20 mM
4	TCEP	natural abundance	4 mM
5	sodium azide	natural abundance	0.05 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	hDlg	[U-100% 13C; U-100% 15N]	0.8 mM
7	E6 protein fragment of HPV type 51	natural abundance	2 mM
8	sodium phosphate	natural abundance	20 mM
9	TCEP	natural abundance	4 mM
10	sodium azide	natural abundance	0.05 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
11	hDlg	natural abundance	3 mM
12	E6 protein fragment of HPV type 51	[U-100% 13C; U-100% 15N]	1.25 mM
13	sodium phosphate	natural abundance	20 mM
14	TCEP	natural abundance	4 mM
15	sodium azide	natural abundance	0.05 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
16	hDlg	natural abundance	3 mM
17	E6 protein fragment of HPV type 51	[U-100% 13C; U-100% 15N]	1.25 mM
18	sodium phosphate	natural abundance	20 mM
19	TCEP	natural abundance	4 mM
20	sodium azide	natural abundance	0.05 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2M3M, Strand ID: A, B Detail

Release date

2012-03-08

Citation

NMR assignment of a PDZ domain in complex with a HPV51 E6 derived N-terminally pyroglutamic acid modified peptide
Mischo, A., Ohlenschlager, O., Ramachandran, R., Gorlach, M.
Biomol. NMR Assign. (2013), 7, 47-49, PubMed 22392342 , DOI 10.1007/s12104-012-9374-1 , Abstract

Related entities 1. hDlg, : 1 : 215 entities Detail