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BMRB: 17952

2LJP

Backbone 1H, 13C, and 15N Chemical Shift Assignments for E.coli Ribonuclease P protein

Authors

Shin, J., Kim, K., Ryu, K., Han, K., Lee, Y., Choi, B.

Assembly

C5 protein monomer

Entity

1. C5 protein monomer (polymer, Thiol state: all free), 119 monomers, 13789.02 Da Detail

MVKLAFPREL RLLTPSQFTF VFQQPQRAGT PQITILGRLN SLGHPRIGLT VAKKNVRRAH ERNRIKRLTR ESFRLRQHEL PAMDFVVVAK KGVADLDNRA LSEALEKLWR RHCRLARGS

Formula weight

13789.02 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.5 %, Completeness: 66.8 %, Completeness (bb): 76.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	66.8 % (973 of 1457)	66.6 % (514 of 772)	61.1 % (343 of 561)	93.5 % (116 of 124)
Backbone	76.6 % (538 of 702)	91.6 % (218 of 238)	60.7 % (213 of 351)	94.7 % (107 of 113)
Sidechain	61.9 % (537 of 868)	55.4 % (296 of 534)	71.8 % (232 of 323)	81.8 % (9 of 11)
Aromatic	9.1 % (8 of 88)	11.4 % (5 of 44)	4.7 % (2 of 43)	100.0 % (1 of 1)
Methyl	68.8 % (99 of 144)	63.9 % (46 of 72)	73.6 % (53 of 72)

1. E.coli Ribonuclease P protein

MVKLAFPREL RLLTPSQFTF VFQQPQRAGT PQITILGRLN SLGHPRIGLT VAKKNVRRAH ERNRIKRLTR ESFRLRQHEL PAMDFVVVAK KGVADLDNRA LSEALEKLWR RHCRLARGS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	E.coli Ribonuclease P protein	[U-95% 13C; U-95% 15N]	0.8 (±0.2) mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	Sodium Phosphate	natural abundance	25 mM
5	Sodium Chloride	natural abundance	200 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LJP, Strand ID: A Detail

Release date

2011-12-08

Citation

Structural analysis of Escherichia coli C5 protein
Shin, J., Kim, K., Ryu, K., Han, K., Lee, Y., Choi, B.
Proteins (2012), 80, 963-967, PubMed 22423363 , Abstract

Related entities 1. C5 protein monomer, : 1 : 4 : 1 : 4 : 96 entities Detail

Interaction partners 1. C5 protein monomer, : 8 interactors Detail

More details for 8 interactors identified by 4 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC