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BMRB: 17957

2LT2

1H, 13C and 15N chemical shifts assignments of BA42 from Bizionia argentinensis

Authors

Smal, C., Aran, M., Gallo, M., Cicero, D.

Assembly

BA42

Entity

1. BA42 (polymer, Thiol state: not present), 145 monomers, 16477.40 Da Detail

MSKIEEFLTA EEEKAIVDAI RDAEKNTSGE IRVHLEKTSE IDVFDRAMDV FHNLKMDNTK LQNGVLIYVA VEDKTFVIYG DKGINDVVSD DFWDTTRNAI QLQFKQGNFK QGLVDGIEKA GMALAKYFPW KKDDIDELPN TISKG

Formula weight

16477.4 Da

Source organism

Bizionia argentinensis

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 94.0 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.0 % (1613 of 1716)	92.6 % (825 of 891)	96.4 % (643 of 667)	91.8 % (145 of 158)
Backbone	98.5 % (853 of 866)	97.6 % (290 of 297)	99.1 % (422 of 426)	98.6 % (141 of 143)
Sidechain	90.8 % (895 of 986)	90.1 % (535 of 594)	94.4 % (356 of 377)	26.7 % (4 of 15)
Aromatic	81.6 % (111 of 136)	86.8 % (59 of 68)	75.8 % (50 of 66)	100.0 % (2 of 2)
Methyl	100.0 % (164 of 164)	100.0 % (82 of 82)	100.0 % (82 of 82)

1. BA42

MSKIEEFLTA EEEKAIVDAI RDAEKNTSGE IRVHLEKTSE IDVFDRAMDV FHNLKMDNTK LQNGVLIYVA VEDKTFVIYG DKGINDVVSD DFWDTTRNAI QLQFKQGNFK QGLVDGIEKA GMALAKYFPW KKDDIDELPN TISKG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 301 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	BA42	[U-100% 13C; U-100% 15N]	0.5 mM
2	potassium phosphate	natural abundance	20 mM
3	PMSF	natural abundance	0.02 mM
4	beta-mercaptoethanol	natural abundance	10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.58 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.58 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.24 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LT2, Strand ID: A Detail

Release date

2011-10-23

Citation

1H, 15N and 13C chemical shift assignments of the BA42 protein of the psychrophilic bacteria Bizionia argentinensis sp. nov
Smal, C., Aran, M., Lanzarotti, E., Papouchado, M., Foti, M., Marti, M.A., Coria, S.H., Vazquez, S.C., Bercovich, A., Mac Cormack, W.P., Turjanski, A.G., Gallo, M., Cicero, D.O.
Biomol. NMR Assign. (2012), 6, 181-183, PubMed 22201035 , DOI 10.1007/s12104-011-9351-0 , Abstract

Related entities 1. BA42, : 1 : 2 : 3 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC