BMRB: 17988

Evolutionary diversification of Mesobuthus {alpha}-scorpion toxins affecting sodium channels.

Authors

Zhu, S., Peigneur, S., Gao, B., Lu, X., Cao, C., Tytgat, J.

Assembly

Mesobuthus {alpha}-scorpion toxins affecting sodium channels

Entity

1. Mesobuthus {alpha}-scorpion toxins affecting sodium channels (polymer, Thiol state: all disulfide bound), 66 monomers, 7179.162 Da Detail

ARDAYIAKPH NCVYECFDAF SSYCNGVCTK NGAKSGYCQI LGTYGNGCWC IALPDNVPIR IPGKCH

Formula weight

7179.162 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS12:SG	1:CYS65:SG
2	disulfide	sing	1:CYS16:SG	1:CYS38:SG
3	disulfide	sing	1:CYS24:SG	1:CYS48:SG
4	disulfide	sing	1:CYS28:SG	1:CYS50:SG

Source organism

Mesobuthus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.0 %, Completeness (bb): 97.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	96.0 % (362 of 377)	96.0 % (362 of 377)
Backbone	97.0 % (131 of 135)	97.0 % (131 of 135)
Sidechain	95.5 % (231 of 242)	95.5 % (231 of 242)
Aromatic	92.5 % (37 of 40)	92.5 % (37 of 40)
Methyl	85.7 % (24 of 28)	85.7 % (24 of 28)

1. entity

ARDAYIAKPH NCVYECFDAF SSYCNGVCTK NGAKSGYCQI LGTYGNGCWC IALPDNVPIR IPGKCH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4.0

#	Name	Isotope labeling	Type	Concentration
1	sodium phosphate	natural abundance		50 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		90 %
4	entity	natural abundance		1 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 2LKB, Strand ID: A Detail

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Release date

2011-11-16

Citation

Evolutionary diversification of Mesobuthus α-scorpion toxins affecting sodium channels
Zhu, S., Peigneur, S., Gao, B., Lu, X., Cao, C., Tytgat, J.
Mol. Cell. Proteomics. (2012), 11, M111.012054-M111.012054, PubMed 21969612 , DOI 10.1074/mcp.M111.012054 , Abstract

Related entities 1. Mesobuthus {alpha}-scorpion toxins affecting sodium channels, : 1 : 2 : 5 : 152 entities Detail

Experiments performed 3 experiments Detail