BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	94.2 % (1240 of 1316)	94.2 % (651 of 691)	95.2 % (476 of 500)	90.4 % (113 of 125)
Backbone	94.6 % (630 of 666)	93.8 % (211 of 225)	95.5 % (317 of 332)	93.6 % (102 of 109)
Sidechain	94.3 % (715 of 758)	94.4 % (440 of 466)	95.7 % (264 of 276)	68.8 % (11 of 16)
Aromatic	89.2 % (66 of 74)	94.6 % (35 of 37)	83.3 % (30 of 36)	100.0 % (1 of 1)
Methyl	100.0 % (118 of 118)	100.0 % (59 of 59)	100.0 % (59 of 59)

1. UUP

GSHMGQQEQY VALKQPAVKK TEEAAAAKAE TVKRSSSKLS YKLQRELEQL PQLLEDLEAK LEALQTQVAD ASFFSQPHEQ TQKVLADMAA AEQELEQAFE RWEYLEALKN GG

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0, Details Uniformly 15N labeled sample

#	Name	Isotope labeling	Concentration
1	UUP	[U-100% 15N]	0.7 mM
2	sodium chloride	natural abundance	170 mM
3	sodium phosphate	natural abundance	30 mM
4	DSS	natural abundance	0.111 mM
5	leupeptine	natural abundance	1 uM
6	pepstatine	natural abundance	1 uM
7	sodium azide	natural abundance	0.02 %
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0, Details Uniformly 15N/13C double labeled sample

#	Name	Isotope labeling	Concentration
10	UUP	[U-100% 13C; U-100% 15N]	0.9 mM
11	sodium chloride	natural abundance	170 mM
12	sodium phosphate	natural abundance	30 mM
13	DSS	natural abundance	0.111 mM
14	leupeptine	natural abundance	1 uM
15	pepstatine A	natural abundance	1 uM
16	sodium azide	natural abundance	0.02 %
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0, Details Uniformly 15N/13C double labeled sample in D2O

#	Name	Isotope labeling	Concentration
19	UUP	[U-100% 13C; U-100% 15N]	0.8 mM
20	sodium chloride	natural abundance	170 mM
21	sodium phosphate	natural abundance	30 mM
22	leupeptine	natural abundance	1 uM
23	pepstatine A	natural abundance	1 uM
24	sodium azide	natural abundance	0.02 %
25	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LW1, Strand ID: A Detail

Release date

2012-01-30

Citation

Secondary structure and NMR resonance assignments of the C-terminal DNA-binding domain of Uup protein
Carlier, L., Haase, A., Burgos Zepeda, M.Y., Dassa, E., Lequin, O.
Biomol. NMR Assign. (2012), 6, 197-200, PubMed 22287065 , DOI 10.1007/s12104-012-9356-3 , Abstract