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BMRB: 18002

2LKO

Structural Basis of Phosphoinositide Binding to Kindlin-2 Pleckstrin Homology Domain in Regulating Integrin Activation

Authors

Liu, J., Fukuda, K., Xu, Z.

Assembly

Kindlin-2

Entity

1. Kindlin-2 (polymer), 138 monomers, 15699.96 Da Detail

GSHMGDITSI PELADYIKVF KPKKLTLKGY KQYWCTFKDT SISCYKSKEE SSGTPAHQMN LRGCEVTPDV NISGQKFNIK LLIPVAEGMN EIWLRCDNEK QYAHWMAACR LASKGKTMAD SSYNLEVQNI LSFLKMQH

2. 4IP (non-polymer), 500.075 Da

Total weight

16200.035 Da

Max. entity weight

15699.96 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 73.4 %, Completeness (bb): 81.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.4 % (1210 of 1649)	77.1 % (665 of 863)	64.8 % (413 of 637)	88.6 % (132 of 149)
Backbone	81.3 % (665 of 818)	91.8 % (256 of 279)	69.0 % (280 of 406)	97.0 % (129 of 133)
Sidechain	70.1 % (674 of 961)	70.0 % (409 of 584)	72.6 % (262 of 361)	18.8 % (3 of 16)
Aromatic	20.7 % (29 of 140)	25.7 % (18 of 70)	14.9 % (10 of 67)	33.3 % (1 of 3)
Methyl	54.6 % (71 of 130)	46.2 % (30 of 65)	63.1 % (41 of 65)

1. Kindlin-2

GSHMGDITSI PELADYIKVF KPKKLTLKGY KQYWCTFKDT SISCYKSKEE SSGTPAHQMN LRGCEVTPDV NISGQKFNIK LLIPVAEGMN EIWLRCDNEK QYAHWMAACR LASKGKTMAD SSYNLEVQNI LSFLKMQH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Kindlin-2	[U-100% 13C; U-100% 15N]	1 mM
2	4IP	natural abundance	2 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
5	Kindlin2-PH	[U-100% 13C; U-100% 15N]	1 mM
6	4IP	natural abundance	2 mM
7	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LKO, Strand ID: A Detail

Release date

2011-11-20

Citation

Structural basis of phosphoinositide binding to kindlin-2 protein pleckstrin homology domain in regulating integrin activation
Liu, J., Fukuda, K., Xu, Z., Ma, Y., Hirbawi, J., Mao, X., Wu, C., Plow, E.F., Qin, J.
J. Biol. Chem. (2011), 286, 43334-43342, PubMed 22030399 , DOI 10.1074/jbc.M111.295352 , Abstract

Related entities 1. Kindlin-2, : 1 : 32 entities Detail

Interaction partners 1. Kindlin-2, : 14 interactors Detail

More details for 14 interactors identified by 7 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC