BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	96.9 % (601 of 620)	97.3 % (324 of 333)	96.6 % (224 of 232)	96.4 % (53 of 55)
Backbone	97.6 % (289 of 296)	99.0 % (98 of 99)	96.6 % (144 of 149)	97.9 % (47 of 48)
Sidechain	96.8 % (361 of 373)	96.6 % (226 of 234)	97.7 % (129 of 132)	85.7 % (6 of 7)
Aromatic	73.3 % (22 of 30)	73.3 % (11 of 15)	78.6 % (11 of 14)	0.0 % (0 of 1)
Methyl	100.0 % (42 of 42)	100.0 % (21 of 21)	100.0 % (21 of 21)

1. FF11-60

GNTKEEAKQA FKELLKEKRV PSNASWEQAM KMIINDPRYS ALAKLSEKKQ

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.8, Details FF11-60

#	Name	Isotope labeling	Concentration
1	sodium acetate	natural abundance	20 mM
2	sodium chloride	natural abundance	50 mM
3	sodium azide	natural abundance	0.05 %
4	FF11-60	[U-100% 13C; U-100% 15N]	1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.8, Details FF11-60 A51L mutant to remove Ala chemical shift ambiguity

#	Name	Isotope labeling	Concentration
7	sodium acetate	natural abundance	20 mM
8	sodium chloride	natural abundance	50 mM
9	sodium azide	natural abundance	0.05 %
10	FF11-60 A51L	[U-100% 13C; U-100% 15N]	1 mM
11	H2O	natural abundance	95 %
12	D2O	natural abundance	5 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.8, Details FF11-60 10% 13C-labelled sample for stereospecific methyl assignment experiment

#	Name	Isotope labeling	Concentration
13	20mM sodium acetate	natural abundance	20 mM
14	50 mM sodium chloride	natural abundance	50 mM
15	0.05% sodium azide	natural abundance	0.05 %
16	FF11-60	[U-10% 13C; U-100% 15N]	1 mM
17	H2O	natural abundance	95 %
18	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	acetate	methyl carbon	25.85 ppm	na	direct	1.0
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	urea	nitrogen	78.98 ppm	na	direct	1.0

Referencing offset: -0.18 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	acetate	methyl carbon	25.85 ppm	na	direct	1.0
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	urea	nitrogen	78.98 ppm	na	direct	1.0

Referencing offset: -0.18 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	acetate	methyl carbon	25.85 ppm	na	direct	1.0
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	urea	nitrogen	78.98 ppm	na	direct	1.0

Referencing offset: 0.04 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	acetate	methyl carbon	25.85 ppm	na	direct	1.0
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	urea	nitrogen	78.98 ppm	na	direct	1.0

Referencing offset: 0.31 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LKS, Strand ID: A Detail

Release date

2011-11-20

Citation

Cross-validation of the structure of a transiently formed and low populated FF domain folding intermediate determined by relaxation dispersion NMR and CS-Rosetta
Barette, J., Velyvis, A., Religa, T.L., Korzhnev, D.M., Kay, L.E.
J. Phys. Chem. B (2012), 116, 6637-6644, PubMed 22148426 , DOI 10.1021/jp209974f , Abstract

Related entities 1. FF11-60, : 1 : 8 : 50 entities Detail

Interaction partners 1. FF11-60, : 96 interactors Detail

More details for 96 interactors identified by 20 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC