BMRBj - BMREntryMaster

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BMRB: 18017

2LKZ

The solution structure of RRM

Authors

Song, Z., Wu, P., Zhang, J., Wu, J., Shi, Y.

Assembly

RRM

Entity

1. RRM (polymer, Thiol state: not present), 95 monomers, 10502.01 Da Detail

MGHHHHHHMD TIILRNIAPH TVVDSIMTAL SPYASLAVNN IRLIKDKQTQ QNRGFAFVQL SSAMDASQLL QILQSLHPPL KIDGKTIGVD FAKSA

Formula weight

10502.01 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.6 %, Completeness: 88.1 %, Completeness (bb): 90.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.1 % (972 of 1103)	90.0 % (512 of 569)	84.7 % (366 of 432)	92.2 % (94 of 102)
Backbone	90.4 % (508 of 562)	91.1 % (173 of 190)	89.7 % (252 of 281)	91.2 % (83 of 91)
Sidechain	86.7 % (548 of 632)	89.4 % (339 of 379)	81.8 % (198 of 242)	100.0 % (11 of 11)
Aromatic	20.0 % (14 of 70)	40.0 % (14 of 35)	0.0 % (0 of 35)
Methyl	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (62 of 62)

1. entity

MGHHHHHHMD TIILRNIAPH TVVDSIMTAL SPYASLAVNN IRLIKDKQTQ QNRGFAFVQL SSAMDASQLL QILQSLHPPL KIDGKTIGVD FAKSA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	RRM	natural abundance	1.2 ~ 1.4 mM
2	sodium phosphate	natural abundance	40 mM
3	sodium chloride	natural abundance	50 mM
4	EDTA	natural abundance	2 mM
5	sodium azide	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4

#	Name	Isotope labeling	Concentration
8	sodium phosphate	natural abundance	40 mM
9	sodium chloride	natural abundance	50 mM
10	EDTA	natural abundance	2 mM
11	sodium azide	natural abundance	1 mM
12	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: -0.27 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: -0.27 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: -0.06 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: 0.14 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LKZ, Strand ID: A Detail

Release date

2012-08-05

Citation

Solution structure of the second RRM domain of RBM5 and its unusual binding characters for different RNA targets
Song, Z., Wu, P., Ji, P., Zhang, J., Gong, Q., Wu, J., Shi, Y.
Biochemistry (2012), 51, 6667-6678, PubMed 22839758 , DOI 10.1021/bi300539t , Abstract

Related entities 1. RRM, : 1 : 112 entities Detail

Interaction partners 1. RRM, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC