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BMRB: 18018

2LL0

NMR structure of the putative ATPase regulatory protein YP_916642.1 from Paracoccus denitrificans

Authors

Serrano, P., Geralt, M., Wuthrich, K., Morales-Rios, E., Zarco-Zavala, M., Garcia-Trejo, J.J., Dutta, S., JCSG, J.

Assembly

YP 916642.1

Entity

1. YP 916642.1 (polymer, Thiol state: not present), 104 monomers, 11668.80 Da Detail

MTTFDDRERA HEAKFAHDAE LNFKAEARRN RLLGEWAAGL LGKTGDDARA YALTVVTSDF DEPGDEDVFR KLAADLEGKA DEETIRAKMV ELRATAREQI ISEI

Formula weight

11668.8 Da

Source organism

Paracoccus denitrificans

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.3 %, Completeness: 83.5 %, Completeness (bb): 87.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.5 % (974 of 1167)	83.3 % (503 of 604)	82.0 % (374 of 456)	90.7 % (97 of 107)
Backbone	87.8 % (546 of 622)	91.1 % (194 of 213)	84.3 % (258 of 306)	91.3 % (94 of 103)
Sidechain	80.6 % (518 of 643)	79.0 % (309 of 391)	82.7 % (205 of 248)	100.0 % (4 of 4)
Aromatic	53.8 % (42 of 78)	53.8 % (21 of 39)	52.6 % (20 of 38)	100.0 % (1 of 1)
Methyl	93.1 % (108 of 116)	93.1 % (54 of 58)	93.1 % (54 of 58)

1. entity

MTTFDDRERA HEAKFAHDAE LNFKAEARRN RLLGEWAAGL LGKTGDDARA YALTVVTSDF DEPGDEDVFR KLAADLEGKA DEETIRAKMV ELRATAREQI ISEI

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	YP_916642.1	[U-98% 13C; U-98% 15N]	1.5 mM
2	sodium chloride	natural abundance	50 mM
3	sodium phosphate	natural abundance	25 mM
4	sodium azide	natural abundance	5 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.45 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.45 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.5 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LL0, Strand ID: A Detail

Release date

2011-11-28

Citation

NMR structure of the putative ATPase regulatory protein YP_916642.1 from Paracoccus denitrificans
Serrano, P., Geralt, M., Wuthrich, K., Dutta, S., Morales-Rios, E., Garcia-Trejo, J.J., Zarco-Zavala, M.
Not known

Related entities 1. YP 916642.1, : 1 : 2 : 2 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC