Solution NMR structure of CaM bound to the eNOS CaM binding domain peptide
ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 59.3 % (1111 of 1874) | 76.7 % (746 of 973) | 29.9 % (217 of 725) | 84.1 % (148 of 176) |
Backbone | 64.4 % (635 of 986) | 87.9 % (298 of 339) | 39.7 % (192 of 484) | 89.0 % (145 of 163) |
Sidechain | 48.5 % (505 of 1042) | 70.8 % (449 of 634) | 13.4 % (53 of 395) | 23.1 % (3 of 13) |
Aromatic | 30.0 % (33 of 110) | 60.0 % (33 of 55) | 0.0 % (0 of 55) | |
Methyl | 46.4 % (77 of 166) | 77.1 % (64 of 83) | 15.7 % (13 of 83) |
1. CaM
ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK2. eNOS
KKTFKEVANA VKISASLSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CaM | [U-99% 13C; U-99% 15N] | 1.0 mM | |
2 | entity_2 | natural abundance | 1.0 mM | |
3 | potassium chloride | natural abundance | 100 mM | |
4 | calcium chloride | natural abundance | 10 mM | |
5 | sodium azide | natural abundance | 0.2 mM | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |