BMRB: 18050

Structure of a bis-naphthalene bound to a thymine-thymine DNA mismatch

Authors

Jourdan, M., Granzhan, A., Guillot, R., Dumy, P., Teulade-Fichou, M.

Assembly

DNA 1

Entity

1. DNA 1 (polymer, Thiol state: not present), 11 monomers, 3428.168 Da Detail

CGTCGTAGTG C

2. DNA 2 (polymer), 11 monomers, 3397.158 Da Detail

GCACTTCGAC G

3. LLJ (non-polymer), 516.717 Da

Total weight

7342.043 Da

Max. entity weight

3428.168 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 74.5 %, Completeness (bb): 67.0 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H	31P
All	74.5 % (178 of 239)	77.9 % (169 of 217)	40.9 % (9 of 22)
Suger, PO4	67.0 % (118 of 176)	70.8 % (109 of 154)	40.9 % (9 of 22)
Nucleobase	95.2 % (60 of 63)	95.2 % (60 of 63)
Aromatic	93.2 % (41 of 44)	93.2 % (41 of 44)
Methyl	100.0 % (5 of 5)	100.0 % (5 of 5)

1. DNA 1

CGTCGTAGTG C

2. DNA 2

GCACTTCGAC G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	DNA_1	natural abundance		1.5 mM
2	DNA_2	natural abundance		1.5 mM
3	2_7-bisnaphthalene	natural abundance		1.5 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 na

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Release date

2012-03-01

Citation

Double threading through DNA: NMR structural study of a bis-naphthalene macrocycle bound to a thymine-thymine mismatch
Jourdan, M., Granzhan, A., Guillot, R., Dumy, P., Teulade-Fichou, M.
Nucleic Acids Res. (2012), 40, 5115-5128, PubMed 22362757 , DOI 10.1093/nar/gks067 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Experiments performed 3 experiments Detail