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BMRB: 18051

2LLK

Solution NMR structure of the N-terminal myb-like 1 domain of the human cyclin-D-binding transcription factor 1 (hDMP1). Northeast Structural Genomics Consortium (NESG) target ID hr8011a.

Authors

Montecchio, M., Lemak, A., Yee, A., Xu, C., Garcia, M., Houliston, S., Min, J., Bellanda, M., Montelione, G., Arrowsmith, C.

Assembly

myb-like1 domain of hDMP1

Entity

1. myb-like1 domain of hDMP1 (polymer, Thiol state: all free), 73 monomers, 8364.252 Da Detail

MHHHHHHSSG RENLYFQGDR NHVGKYTPEE IEKLKELRIK HGNDWATIGA ALGRSASSVK DRCRLMKDTC NTG

Formula weight

8364.252 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 76.7 %, Completeness: 72.8 %, Completeness (bb): 75.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.8 % (609 of 837)	72.7 % (319 of 439)	72.5 % (232 of 320)	74.4 % (58 of 78)
Backbone	75.5 % (329 of 436)	75.7 % (115 of 152)	75.0 % (159 of 212)	76.4 % (55 of 72)
Sidechain	70.7 % (330 of 467)	71.1 % (204 of 287)	70.7 % (123 of 174)	50.0 % (3 of 6)
Aromatic	31.4 % (22 of 70)	34.3 % (12 of 35)	29.4 % (10 of 34)	0.0 % (0 of 1)
Methyl	92.9 % (52 of 56)	92.9 % (26 of 28)	92.9 % (26 of 28)

1. entity 1

MHHHHHHSSG RENLYFQGDR NHVGKYTPEE IEKLKELRIK HGNDWATIGA ALGRSASSVK DRCRLMKDTC NTG

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity 1	[U-13C; U-15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	NaCl	natural abundance	400 mM
4	ZnSO4	natural abundance	0.01 mM
5	DTT	natural abundance	10 mM
6	benzamidine	natural abundance	1 mM
7	NaN3	natural abundance	0.01 %
8	H2O	natural abundance	95 %
9	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.54 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LLK, Strand ID: A Detail

Release date

2012-01-05

Citation

Solution NMR structure of the N-terminal myb-like 1 domain of the human cyclin-D-binding transcription factor 1 (hDMP1)
Montecchio, M., Lemak, A., Yee, A., Xu, C., Garcia, M., Houliston, S., Min, J., Bellanda, M., Arrowsmith, C., Montelione, G.
Not known

Related entities 1. myb-like1 domain of hDMP1, : 1 : 11 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC