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BMRB: 18053

2LLL

Solution NMR structure of C-terminal globular domain of human Lamin-B2. Northeast Structural Genomics Consortium target HR8546A.

Authors

Lemak, A., Yee, A., Houliston, S., Garcia, M., Xu, C., Min, J., Arrowsmith, C.

Assembly

hs499

Entity

1. hs499 (polymer, Thiol state: not present), 139 monomers, 15453.05 Da Detail

MHHHHHHSSG RENLYFQGSA SGSVSIEEID LEGKFVQLKN NSDKDQSLGN WRIKRQVLEG EEIAYKFTPK YILRAGQMVT VWAAGAGVAH SPPSTLVWKG QSSWGTGESF RTVLVNADGE EVAMRTVKKS SVMRENENG

Formula weight

15453.05 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.2 %, Completeness: 81.4 %, Completeness (bb): 87.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.4 % (1295 of 1590)	80.5 % (665 of 826)	81.2 % (496 of 611)	87.6 % (134 of 153)
Backbone	87.1 % (721 of 828)	86.9 % (251 of 289)	87.1 % (351 of 403)	87.5 % (119 of 136)
Sidechain	77.0 % (683 of 887)	77.1 % (414 of 537)	76.3 % (254 of 333)	88.2 % (15 of 17)
Aromatic	48.6 % (68 of 140)	48.6 % (34 of 70)	45.5 % (30 of 66)	100.0 % (4 of 4)
Methyl	92.3 % (120 of 130)	92.3 % (60 of 65)	92.3 % (60 of 65)

1. hs499

MHHHHHHSSG RENLYFQGSA SGSVSIEEID LEGKFVQLKN NSDKDQSLGN WRIKRQVLEG EEIAYKFTPK YILRAGQMVT VWAAGAGVAH SPPSTLVWKG QSSWGTGESF RTVLVNADGE EVAMRTVKKS SVMRENENG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	hs499	[U-13C; U-15N]	0.5 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	450 mM
4	ZnSO4	natural abundance	10 uM
5	DTT	natural abundance	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.65 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LLL, Strand ID: A Detail

Release date

2011-12-05

Citation

NMR solution structure of c-terminal globular domain of human Lamin-B2
Lemak, A., Yee, A., Houliston, S., Garcia, M., Xu, C., Min, J., Arrowsmith, C.
Not known

Related entities 1. hs499, : 1 : 2 : 47 entities Detail

Interaction partners 1. hs499, : 30 interactors Detail

More details for 30 interactors identified by 9 citations

Experiments performed 9 experiments Detail

1 3D HNCO