BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	85.8 % (1257 of 1465)	87.7 % (664 of 757)	81.8 % (473 of 578)	92.3 % (120 of 130)
Backbone	94.8 % (696 of 734)	94.1 % (238 of 253)	95.4 % (349 of 366)	94.8 % (109 of 115)
Sidechain	79.4 % (671 of 845)	84.5 % (426 of 504)	71.8 % (234 of 326)	73.3 % (11 of 15)
Aromatic	38.6 % (51 of 132)	77.3 % (51 of 66)	0.0 % (0 of 65)	0.0 % (0 of 1)
Methyl	90.1 % (137 of 152)	89.5 % (68 of 76)	90.8 % (69 of 76)

1. apoCuA polypeptide

MVIPAGKLER VDPTTVRQEG PWADPAQAVV QTGPNQYTVY VLAFAFGYQP NPIEVPQGAE IVFKITSPDV IHGFHVEGTN INVEVLPGEV STVRYTFKRP GEYRIICNQY CGLGHQNMFG TIVVKE

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 100 mM potassium phosphate pH 6, 2 mM DTT

#	Name	Isotope labeling	Concentration
1	apoCuA_polypeptide	[U-99% 13C; U-99% 15N]	0.8 ~ 1.0 mM
2	potassium phosphate	natural abundance	100 mM
3	DTT	natural abundance	2 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 100 mM potassium phosphate pH 6, 2 mM DTT

#	Name	Isotope labeling	Concentration
6	apoCuA_polypeptide	[U-99% 15N]	0.8 ~ 1.0 mM
7	potassium phosphate	natural abundance	100 mM
8	DTT	natural abundance	2 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 100 mM potassium phosphate pH 6, 2 mM DTT

#	Name	Isotope labeling	Concentration
11	apoCuA_polypeptide	natural abundance	0.8 ~ 1.0 mM
12	potassium phosphate	natural abundance	100 mM
13	DTT	natural abundance	2 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %

Chem. Shift Complete2

Sequence coverage: 35.7 %, Completeness: 50.6 %, Completeness (bb): 58.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	50.6 % (1483 of 2930)	50.5 % (764 of 1514)	48.3 % (558 of 1156)	61.9 % (161 of 260)
Backbone	58.0 % (852 of 1468)	57.5 % (291 of 506)	56.3 % (412 of 732)	64.8 % (149 of 230)
Sidechain	45.2 % (764 of 1690)	46.9 % (473 of 1008)	42.8 % (279 of 652)	40.0 % (12 of 30)
Aromatic	20.1 % (53 of 264)	40.2 % (53 of 132)	0.0 % (0 of 130)	0.0 % (0 of 2)
Methyl	56.3 % (171 of 304)	55.3 % (84 of 152)	57.2 % (87 of 152)

1. apoCuA polypeptide

MVIPAGKLER VDPTTVRQEG PWADPAQAVV QTGPNQYTVY VLAFAFGYQP NPIEVPQGAE IVFKITSPDV IHGFHVEGTN INVEVLPGEV STVRYTFKRP GEYRIICNQY CGLGHQNMFG TIVVKE

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 100 mM potassium phosphate pH 6, 2 mM DTT

#	Name	Isotope labeling	Concentration
1	apoCuA_polypeptide	[U-99% 13C; U-99% 15N]	0.8 ~ 1.0 mM
2	potassium phosphate	natural abundance	100 mM
3	DTT	natural abundance	2 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 100 mM potassium phosphate pH 6, 2 mM DTT

#	Name	Isotope labeling	Concentration
6	apoCuA_polypeptide	[U-99% 15N]	0.8 ~ 1.0 mM
7	potassium phosphate	natural abundance	100 mM
8	DTT	natural abundance	2 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 100 mM potassium phosphate pH 6, 2 mM DTT

#	Name	Isotope labeling	Concentration
11	apoCuA_polypeptide	natural abundance	0.8 ~ 1.0 mM
12	potassium phosphate	natural abundance	100 mM
13	DTT	natural abundance	2 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2LLN, Strand ID: A Detail

Release date

2012-06-18

Citation

Flexibility of the metal-binding region in apo-cupredoxins
Zaballa, M., Abriata, L.A., Donaire, A., Vila, A.J.
Proc. Natl. Acad. Sci. U. S. A. (2012), 109, 9254-9259, PubMed 22645370 , DOI 10.1073/pnas.1119460109 , Abstract

1 2D 1H-15N HSQC