Solution structure of the N-terminal domain of human polypeptide chain release factor eRF1
MADDPSAADR NVEIWKIKKL IKSLEAARGN GTSMISLIIP PKDQISRVAK MLADEFGTAS NIKSRVNRLS VLGAITSVQQ RLKLYNKVPP NGLVVYCGTI VTEEGKEKKV NIDFEPFKPI NTSLYLCDNK FHTEALTALL SDLEHHHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.3 % (1520 of 1762) | 90.5 % (830 of 917) | 78.1 % (538 of 689) | 97.4 % (152 of 156) |
Backbone | 95.7 % (848 of 886) | 95.7 % (287 of 300) | 95.3 % (422 of 443) | 97.2 % (139 of 143) |
Sidechain | 79.0 % (805 of 1019) | 87.4 % (539 of 617) | 65.3 % (254 of 389) | 92.3 % (12 of 13) |
Aromatic | 39.4 % (41 of 104) | 75.0 % (39 of 52) | 2.0 % (1 of 51) | 100.0 % (1 of 1) |
Methyl | 93.0 % (173 of 186) | 97.8 % (91 of 93) | 88.2 % (82 of 93) |
1. NeRF1
MADDPSAADR NVEIWKIKKL IKSLEAARGN GTSMISLIIP PKDQISRVAK MLADEFGTAS NIKSRVNRLS VLGAITSVQQ RLKLYNKVPP NGLVVYCGTI VTEEGKEKKV NIDFEPFKPI NTSLYLCDNK FHTEALTALL SDLEHHHHHHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | NeRF1 | [U-99% 13C; U-99% 15N] | 1 mM | |
2 | sodium chloride | natural abundance | 25 mM | |
3 | sodium phosphate | natural abundance | 10 mM | |
4 | beta-mercaptoethanol | natural abundance | 2 mM | |
5 | sodium azide | natural abundance | 0.01 % | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | NeRF1 | [U-99% 13C; U-99% 15N] | 1 mM | |
9 | sodium chloride | natural abundance | 25 mM | |
10 | sodium phosphate | natural abundance | 10 mM | |
11 | beta-mercaptoethanol | natural abundance | 2 mM | |
12 | sodium azide | natural abundance | 0.01 % | |
13 | D2O | natural abundance | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | NeRF1 | natural abundance | 1 mM | |
15 | sodium chloride | natural abundance | 25 mM | |
16 | sodium phosphate | natural abundance | 10 mM | |
17 | beta-mercaptoethanol | natural abundance | 2 mM | |
18 | sodium azide | natural abundance | 0.01 % | |
19 | D2O | natural abundance | 100 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | NeRF1 | [U-99% 15N] | 1 mM | |
21 | sodium chloride | natural abundance | 25 mM | |
22 | sodium phosphate | natural abundance | 10 mM | |
23 | beta-mercaptoethanol | natural abundance | 2 mM | |
24 | sodium azide | natural abundance | 0.01 % | |
25 | H2O | natural abundance | 90 % | |
26 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
27 | NeRF1 | [U-99% 15N] | 1 mM | |
28 | sodium chloride | natural abundance | 25 mM | |
29 | sodium phosphate | natural abundance | 10 mM | |
30 | beta-mercaptoethanol | natural abundance | 2 mM | |
31 | sodium azide | natural abundance | 0.01 % | |
32 | C12E5 | natural abundance | 5.00 % | |
33 | hexanol | natural abundance | 1.068 % | |
34 | H2O | natural abundance | 90 % | |
35 | D2O | natural abundance | 10 % |