BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	78.8 % (1182 of 1500)	77.6 % (612 of 789)	79.2 % (465 of 587)	84.7 % (105 of 124)
Backbone	88.1 % (643 of 730)	86.5 % (212 of 245)	88.4 % (327 of 370)	90.4 % (104 of 115)
Sidechain	73.1 % (651 of 890)	73.5 % (400 of 544)	74.2 % (250 of 337)	11.1 % (1 of 9)
Aromatic	54.8 % (34 of 62)	90.3 % (28 of 31)	19.4 % (6 of 31)
Methyl	82.7 % (129 of 156)	87.2 % (68 of 78)	78.2 % (61 of 78)

1. AF4-Af9 fusion

TRLPLPLRDT KLLSPLRDTP PPQSLMVKIT LDLLSRIPQP PGKPMGSDKQ IKNGECDKAY LDELVELHRR LMTLRERHIL QQIVNLIEET GHFHITNTTF DFDLCSLDKT TVRKLQSYLE TSGTS

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0, Details 400uM AF4-AF9 fusion

#	Name	Isotope labeling	Concentration
1	Bis-Tris	natural abundance	9.3 mM
2	MES	natural abundance	15.8 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	D2O	D-99%	5 %
6	AF4-AF9_fus	[U-100% 13C; U-100% 15N]	400 uM
7	H2O	natural abundance	95 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0, Details 2mM AF4-AF9 coexpressed

#	Name	Isotope labeling	Concentration
8	MES	natural abundance	15.8 mM
9	Bis-Tris	natural abundance	9.3 mM
10	sodium chloride	natural abundance	100 mM
11	DTT	natural abundance	1 mM
12	D2O	D-99%	5 %
13	AF4-AF9_fus	[U-100% 13C; U-100% 15N]	2 mM
14	H2O	natural abundance	95 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0, Details 400uM AF4-AF9 fusion

#	Name	Isotope labeling	Concentration
15	Bis-Tris	natural abundance	9.3 mM
16	MES	natural abundance	15.8 mM
17	sodium chloride	natural abundance	100 mM
18	DTT	natural abundance	1 mM
19	D2O	D-99%	5 %
20	AF4-AF9_fus	[U-100% 13C; U-100% 15N]	400 uM
21	(3-acrylamidopropyl)-trimethylammonium chloride	natural abundance	3.5 %
22	acrylic acid	natural abundance	3.5 %
23	H2O	natural abundance	95 %

Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0, Details 400uM AF4-AF9 fusion

#	Name	Isotope labeling	Concentration
24	Bis-Tris	natural abundance	9.3 mM
25	MES	natural abundance	15.8 mM
26	sodium chloride	natural abundance	100 mM
27	DTT	natural abundance	1 mM
28	D2O	D-99%	5 %
29	AF4-AF9_fus	[U-100% 13C; U-100% 15N]	400 uM
30	(3-acrylamidopropyl)-trimethylammonium chloride	natural abundance	3.5 %
31	acrylamide	natural abundance	3.5 %
32	H2O	natural abundance	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.75 ppm	internal	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	water	protons	4.75 ppm	internal	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.75 ppm	internal	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	water	protons	4.75 ppm	internal	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.75 ppm	internal	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	water	protons	4.75 ppm	internal	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.75 ppm	internal	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	water	protons	4.75 ppm	internal	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LM0, Strand ID: A Detail

Release date

2013-01-06

Citation

Leukemia fusion target AF9 is an intrinsically disordered transcriptional regulator that recruits multiple partners via coupled folding and binding
Leach, B.I., Kuntimaddi, A., Schmidt, C.R., Cierpicki, T., Johnson, S.A., Bushweller, J.H.
Structure (2013), 21, 176-183, PubMed 23260655 , DOI 10.1016/j.str.2012.11.011 , Abstract

Entries sharing articles Swiss-Prot: 2 entries Detail

  Swiss-Prot: P51825 released on 1996-10-01
    Title AFF1_HUMAN Entity AF4/FMR2 family member 1
  Swiss-Prot: P42568 released on 1995-11-01
    Title AF9_HUMAN Entity Protein AF-9

Related entities 1. AF4-Af9 fusion, : 1 : 2 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC