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BMRB: 18096

2LM2

NMR structures of the transmembrane domains of the AChR b2 subunit

Authors

Bondarenko, V., Mowrey, D., Tillman, T., Cui, T., Liu, L.T., Xu, Y., Tang, P.

Assembly

nAChR

Entity

1. nAChR (polymer, Thiol state: all free), 137 monomers, 15078.72 Da Detail

SNAEEEPLFY TINLIIPCVL ITSLAILVFY LPSDCGEKMT LCISVLLALT VFLLLISKIV PPTSSDSPSV GEYLMFTMVL VTFSIVTSVC VLNVHHRSPE THTGGGGGID RLFLWIFVFV CVFGTIGMFL QPLFQEE

Formula weight

15078.72 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 82.3 %, Completeness (bb): 94.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.3 % (1325 of 1610)	78.9 % (642 of 814)	84.0 % (555 of 661)	94.8 % (128 of 135)
Backbone	94.7 % (763 of 806)	94.9 % (261 of 275)	93.5 % (376 of 402)	97.7 % (126 of 129)
Sidechain	73.0 % (680 of 932)	70.7 % (381 of 539)	76.7 % (297 of 387)	33.3 % (2 of 6)
Aromatic	27.2 % (43 of 158)	29.1 % (23 of 79)	25.6 % (20 of 78)	0.0 % (0 of 1)
Methyl	86.1 % (186 of 216)	86.1 % (93 of 108)	86.1 % (93 of 108)

1. entity

SNAEEEPLFY TINLIIPCVL ITSLAILVFY LPSDCGEKMT LCISVLLALT VFLLLISKIV PPTSSDSPSV GEYLMFTMVL VTFSIVTSVC VLNVHHRSPE THTGGGGGID RLFLWIFVFV CVFGTIGMFL QPLFQEE

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 4.65

#	Name	Isotope labeling	Concentration
1	nAChR	[U-100% 13C; U-100% 15N]	0.25 mM
2	sodium acetate	natural abundance	5 mM
3	LDAO	natural abundance	1.5 %
4	DSS	natural abundance	0.1 mM
5	D2O	[U-100% 2H]	5 %
6	sodium chloride	natural abundance	10 mM
7	beta-mercaptoethanol	natural abundance	20 mM
8	H2O	natural abundance	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.32 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LM2, Strand ID: A Detail

Release date

2013-02-14

Citation

NMR structures of the transmembrane domains of the α4β2 nAChR
Bondarenko, V., Mowrey, D., Tillman, T., Cui, T., Liu, L., Xu, Y., Tang, P.
Biochim. Biophys. Acta (2012), 1818, 1261-1268, PubMed 22361591 , DOI 10.1016/j.bbamem.2012.02.008 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 2 entries Detail

  BMRB: 18093 released on 2012-03-01
    Title NMR structures of the transmembrane domains of the nAChR a4 subunit
  Swiss-Prot: P43681 released on 1995-11-01
    Title ACHA4_HUMAN Entity Neuronal acetylcholine receptor subunit alpha-4
  Swiss-Prot: P17787 released on 1990-08-01
    Title ACHB2_HUMAN Entity Neuronal acetylcholine receptor subunit beta-2

Related entities 1. nAChR, : 1 : 58 entities Detail

Experiments performed 8 experiments Detail

1 3D 1H-15N NOESY