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BMRB: 18098

2LM4

Solution NMR Structure of mitochondrial succinate dehydrogenase assembly factor 2 from Saccharomyces cerevisiae, Northeast Structural Genomics Consortium Target YT682A

Authors

Eletsky, A., Lee, H., Lee, D., Kohan, E., Hamilton, K., Acton, T.B., Xiao, R., Everett, J.K., Prestegard, J.H., Montelione, G.T., Szyperski, T.

Assembly

YT682A

Entity

1. YT682A (polymer, Thiol state: not present), 109 monomers, 13166.82 Da Detail

MGHHHHHHSH MIKRTNEPLD KKRARLIYQS RKRGILETDL LLSGFAAKYL KKMNEEELEE YDSLLNELDW DIYYWATKNF KTSPLPDKWA NSKLLKQLQE FSENKEKEI

Formula weight

13166.82 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

RDCs, assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 92.9 %, Completeness (bb): 92.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.9 % (1281 of 1379)	92.6 % (676 of 730)	93.2 % (495 of 531)	93.2 % (110 of 118)
Backbone	92.6 % (600 of 648)	91.7 % (200 of 218)	93.2 % (302 of 324)	92.5 % (98 of 106)
Sidechain	93.3 % (781 of 837)	93.0 % (476 of 512)	93.6 % (293 of 313)	100.0 % (12 of 12)
Aromatic	82.1 % (110 of 134)	82.1 % (55 of 67)	81.3 % (52 of 64)	100.0 % (3 of 3)
Methyl	100.0 % (98 of 98)	100.0 % (49 of 49)	100.0 % (49 of 49)

1. YT682A

MGHHHHHHSH MIKRTNEPLD KKRARLIYQS RKRGILETDL LLSGFAAKYL KKMNEEELEE YDSLLNELDW DIYYWATKNF KTSPLPDKWA NSKLLKQLQE FSENKEKEI

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM [U-100% 13C; U-100% 15N] YT682A, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	YT682A	[U-100% 13C; U-100% 15N]	1.0 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM [5% 13C; U-100% 15N] YT682A, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
8	YT682A	[5% 13C; U-100% 15N]	1.1 mM
9	MES	natural abundance	20 mM
10	sodium chloride	natural abundance	100 mM
11	calcium chloride	natural abundance	5 mM
12	DTT	natural abundance	10 mM
13	sodium azide	natural abundance	0.02 %
14	DSS	natural abundance	50 uM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [5% 13C; U-100% 15N] YT682A aligned in PEG, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
15	YT682A	[5% 13C; U-100% 15N]	0.7 mM
16	MES	natural abundance	20 mM
17	sodium chloride	natural abundance	100 mM
18	calcium chloride	natural abundance	5 mM
19	DTT	natural abundance	10 mM
20	sodium azide	natural abundance	0.02 %
21	DSS	natural abundance	50 uM
22	C12E5 PEG	natural abundance	4 %
23	hexanol	natural abundance	4 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM [5% 13C; U-100% 15N] YT682A aligned in positively charged compressed gel, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
24	YT682A	[5% 13C; U-100% 15N]	1.1 mM
25	MES	natural abundance	20 mM
26	sodium chloride	natural abundance	100 mM
27	calcium chloride	natural abundance	5 mM
28	DTT	natural abundance	10 mM
29	sodium azide	natural abundance	0.02 %
30	DSS	natural abundance	50 uM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LM4, Strand ID: A Detail

RDC

152 RDC values in 2 lists
Field strength (¹H) 599.5202 MHz, Pressure 1 atm, Temperature 298 K, pH 6.5 Detail

Release date

2011-12-04

Related entities 1. YT682A, : 1 : 3 : 61 entities Detail

Interaction partners 1. YT682A, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC