BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	74.2 % (363 of 489)	79.9 % (214 of 268)	62.6 % (109 of 174)	85.1 % (40 of 47)
Backbone	79.8 % (201 of 252)	96.6 % (84 of 87)	61.6 % (77 of 125)	100.0 % (40 of 40)
Sidechain	71.7 % (198 of 276)	71.8 % (130 of 181)	77.3 % (68 of 88)	0.0 % (0 of 7)
Aromatic	0.0 % (0 of 12)	0.0 % (0 of 6)	0.0 % (0 of 5)	0.0 % (0 of 1)
Methyl	91.7 % (11 of 12)	100.0 % (6 of 6)	83.3 % (5 of 6)

1. ctRAGE

MWQRRQRRGE ERKAPENQEE EEERAELNQS EEPEAGESST GGP

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 10 mM potassium phosphate [pH 6.5], 100 mM NaCl, 0.02% (w/v) NaN3, in 90%H2O/10%D2O

#	Name	Isotope labeling	Concentration
1	ctRAGE	[U-99% 13C; U-99% 15N]	300 uM
2	D2O	[U-100% 2H]	10 %
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	sodium azide	natural abundance	0.02 %
6	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.98 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.98 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LMB, Strand ID: A Detail

Release date

2011-12-19

Citation

Free radicals and antioxidant systems in health and disease
Sinclair, A.J., Barnett, A.H., Lunec, J.
Br. J. Hosp. Med. (1990), 43, 334-344, PubMed 2194616 , DOI: , Abstract

Related entities 1. C-terminal RAGE (ctRAGE), : 1 : 1 : 2 : 8 entities Detail

Interaction partners 1. C-terminal RAGE (ctRAGE), : 16 interactors Detail

More details for 16 interactors identified by 11 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 10 mM potassium phosphate [pH 6.5], 100 mM NaCl, 0.02% (w/v) NaN3, in 90%H2O/10%D2O

#	Name	Isotope labeling	Concentration
1	ctRAGE	[U-99% 13C; U-99% 15N]	300 uM
2	D2O	[U-100% 2H]	10 %
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	sodium azide	natural abundance	0.02 %
6	H2O	natural abundance	90 %

2 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ctRAGE	[U-99% 13C; U-99% 15N]	300 uM
2	D2O	[U-100% 2H]	10 %
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	sodium azide	natural abundance	0.02 %
6	H2O	natural abundance	90 %

3 3D HNCO

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ctRAGE	[U-99% 13C; U-99% 15N]	300 uM
2	D2O	[U-100% 2H]	10 %
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	sodium azide	natural abundance	0.02 %
6	H2O	natural abundance	90 %

4 3D HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ctRAGE	[U-99% 13C; U-99% 15N]	300 uM
2	D2O	[U-100% 2H]	10 %
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	sodium azide	natural abundance	0.02 %
6	H2O	natural abundance	90 %

5 3D HNCA

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ctRAGE	[U-99% 13C; U-99% 15N]	300 uM
2	D2O	[U-100% 2H]	10 %
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	sodium azide	natural abundance	0.02 %
6	H2O	natural abundance	90 %

6 3D HN(CO)CA

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ctRAGE	[U-99% 13C; U-99% 15N]	300 uM
2	D2O	[U-100% 2H]	10 %
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	sodium azide	natural abundance	0.02 %
6	H2O	natural abundance	90 %

7 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ctRAGE	[U-99% 13C; U-99% 15N]	300 uM
2	D2O	[U-100% 2H]	10 %
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	sodium azide	natural abundance	0.02 %
6	H2O	natural abundance	90 %

8 3D 1H-15N TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ctRAGE	[U-99% 13C; U-99% 15N]	300 uM
2	D2O	[U-100% 2H]	10 %
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	sodium azide	natural abundance	0.02 %
6	H2O	natural abundance	90 %

9 3D 1H-13C NOESY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ctRAGE	[U-99% 13C; U-99% 15N]	300 uM
2	D2O	[U-100% 2H]	10 %
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	sodium azide	natural abundance	0.02 %
6	H2O	natural abundance	90 %

10 3D HCCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	ctRAGE	[U-99% 13C; U-99% 15N]	300 uM
2	D2O	[U-100% 2H]	10 %
3	potassium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	100 mM
5	sodium azide	natural abundance	0.02 %
6	H2O	natural abundance	90 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_18110_2lmb.nef
Input source #2: Coordindates	2lmb.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40---
MWQRRQRRGEERKAPENQEEEEERAELNQSEEPEAGESSTGGP
|||||||||||||||||||||||||||||||||||||||||||
MWQRRQRRGEERKAPENQEEEEERAELNQSEEPEAGESSTGGP

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	43	0	0	100.0

Content subtype: combined_18110_2lmb.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	361		93.0 (chain: A, length: 43)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	Warning	208 (1)	noe	32.6 (chain: A, length: 43)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_3	OK	114 (114)	.	34.9 (chain: A, length: 43)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 43, Sequence coverage: 93.0%
- Total number of assignments
  - ¹H chemical shifts: 214
  - ¹³C chemical shifts: 107
  - ¹⁵N chemical shifts: 40
- Completeness of assignments
  - Entity_assemble_ID: A
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	268	213	79.5
¹³C chemical shifts	174	107	61.5
¹⁵N chemical shifts	53	40	75.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	87	83	95.4
¹³C chemical shifts	86	40	46.5
¹⁵N chemical shifts	40	40	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	181	130	71.8
¹³C chemical shifts	88	67	76.1
¹⁵N chemical shifts	13	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	7	6	85.7
¹³C chemical shifts	7	5	71.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	6	0	0.0
¹³C chemical shifts	5	0	0.0
¹⁵N chemical shifts	1	0	0.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 43, Sequence coverage: 32.6%
- Total number of restraints: 208
- Weight of restraints: 1.0 (208)
- Potential type of restraints: upper-bound-parabolic (208)
- Range of distance target values: 2.0, 7.57 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 43, Sequence coverage: 34.9%
- Total number of restraints: 114
- Total number of restraints per polymer type: 114
- Weight of restraints: 1.0 (114)
- Potential type of restraints: square-well-parabolic (114)
- Plot of Phi-Psi angle restraints
- Plot of Chi1-Chi2 angle restraints
- Dihedral angle restraints per residue

Keywords cytosolic tail, Protein, Receptor for Advanced Glycation End Products (RAGE), Signal Transduction Receptor

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Found 1 Document (0.721 seconds)

BMRB: 18110

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. C-terminal RAGE (ctRAGE), : 1 : 1 : 2 : 8 entities Detail

Interaction partners 1. C-terminal RAGE (ctRAGE), : 16 interactors Detail

Experiments performed 10 experiments Detail

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NMR combined restraints 4 contents Detail