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BMRB: 18125

2MF6

Resonance assignments of the 56 kDa chimeric avidin in the biotin-bound and free forms

Authors

Tossavainen, H., Helppolainen, S.H., Maatta, J.AE., Pihlajamaa, T., Hytonen, V.P., Kulomaa, M.S., Permi, P.

Assembly

avidin

Entity

1. avidin (polymer, Thiol state: all disulfide bound), 129 monomers, 14383.11 × 2 Da Detail

QTVARKCSLT GKWTNDLGSN MTIGAVNSRG EFTGTYITAV ADNPGNITLS PLLGIQHKRA SQPTFGFTVN WKFSESTTVF TGQCFIDRNG KEVLKTMWLL RSSVNDIGDD WKATRVGYNI FTRLRTQKE

Total weight

28766.22 Da

Max. entity weight

14383.11 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS84:SG

Source organism

Gallus gallus

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 85.0 %, Completeness (bb): 81.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.0 % (1271 of 1496)	91.6 % (707 of 772)	75.9 % (440 of 580)	86.1 % (124 of 144)
Backbone	81.8 % (628 of 768)	94.4 % (252 of 267)	69.1 % (259 of 375)	92.9 % (117 of 126)
Sidechain	88.3 % (746 of 845)	89.1 % (450 of 505)	89.8 % (289 of 322)	38.9 % (7 of 18)
Aromatic	90.6 % (125 of 138)	95.7 % (66 of 69)	84.6 % (55 of 65)	100.0 % (4 of 4)
Methyl	96.5 % (137 of 142)	97.2 % (69 of 71)	95.8 % (68 of 71)

1. avidin

QTVARKCSLT GKWTNDLGSN MTIGAVNSRG EFTGTYITAV ADNPGNITLS PLLGIQHKRA SQPTFGFTVN WKFSESTTVF TGQCFIDRNG KEVLKTMWLL RSSVNDIGDD WKATRVGYNI FTRLRTQKE

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 343 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	avidin	[U-13C; U-15N]	0.7 ~ 1.2 mM
2	d-biotin	natural abundance	0.7 ~ 1.2 mM
3	H2O	natural abundance	93 %
4	D2O	natural abundance	7 %

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 343 K, pH 6.5

#	Name	Isotope labeling	Concentration
5	avidin	[U-13C; U-15N]	0.7 ~ 1.2 mM
6	H2O	natural abundance	93 %
7	D2O	natural abundance	7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	175.2 ppm	na	indirect	0.2514495
¹H	water	protons	4.4 ppm	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	117.81 ppm	na	indirect	0.1013291

Referencing offset: -0.65 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	175.2 ppm	na	indirect	0.2514495
¹H	water	protons	4.4 ppm	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	117.81 ppm	na	indirect	0.1013291

Referencing offset: -0.65 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	175.2 ppm	na	indirect	0.2514495
¹H	water	protons	4.4 ppm	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	117.81 ppm	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2MF6, Strand ID: A, B, C, D Detail

Release date

2012-03-08

Citation

Resonance assignments of the 56 kDa chimeric avidin in the biotin-bound and free forms
Tossavainen, H., Helppolainen, S.H., Maatta, J.A.E., Pihlajamaa, T., Hytonen, V.P., Kulomaa, M.S., Permi, P.
Biomol. NMR Assign. (2013), 7, 35-38, PubMed 22392339 , DOI 10.1007/s12104-012-9371-4 , Abstract

Related entities 1. avidin, : 1 : 1 : 112 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC