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BMRB: 18151

2LWJ

1H, 13C, and 15N chemical shift assignments for CdnL from Myxoccoccus xanthus

Authors

Jimenez, M., Padmanabhan, S., Mirassou, Y.

Assembly

CdnL

Entity

1. CdnL (polymer, Thiol state: all free), 167 monomers, 18999.60 Da Detail

GSHMQTSFKT GDKAVYPGQG VGEVMGIEHT EVAGQRQSFY VLRILENGMR IMIPINKVGS VGLREIISEE DVKQVYSILK EKDISVDSTT WNRRYREYME KIKTGSVFEI AEVLRDLYLL KGDKDLSFGE RKMLDTARSL LIKELSLAKD CSEDEIESDL KKIFNLA

Formula weight

18999.6 Da

Source organism

Myxococcus xanthus

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 81.8 %, Completeness (bb): 86.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.8 % (1621 of 1981)	86.8 % (899 of 1036)	72.7 % (560 of 770)	92.6 % (162 of 175)
Backbone	86.6 % (864 of 998)	91.3 % (315 of 345)	81.4 % (397 of 488)	92.1 % (152 of 165)
Sidechain	75.0 % (853 of 1137)	84.5 % (584 of 691)	59.4 % (259 of 436)	100.0 % (10 of 10)
Aromatic	55.9 % (66 of 118)	94.9 % (56 of 59)	15.5 % (9 of 58)	100.0 % (1 of 1)
Methyl	92.3 % (179 of 194)	95.9 % (93 of 97)	88.7 % (86 of 97)

1. CdnL

GSHMQTSFKT GDKAVYPGQG VGEVMGIEHT EVAGQRQSFY VLRILENGMR IMIPINKVGS VGLREIISEE DVKQVYSILK EKDISVDSTT WNRRYREYME KIKTGSVFEI AEVLRDLYLL KGDKDLSFGE RKMLDTARSL LIKELSLAKD CSEDEIESDL KKIFNLA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CdnL	natural abundance	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	sodium azide	natural abundance	0.05 %
5	beta-mercaptoethanol	natural abundance	2 mM
6	DSS	natural abundance	0.5 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	CdnL	natural abundance	1 mM
8	sodium phosphate	natural abundance	50 mM
9	sodium chloride	natural abundance	100 mM
10	sodium azide	natural abundance	0.05 %
11	beta-mercaptoethanol	natural abundance	2 mM
12	DSS	natural abundance	0.5 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
13	CdnL	[U-100% 13C; U-100% 15N]	1 mM
14	sodium phosphate	natural abundance	50 mM
15	sodium chloride	natural abundance	100 mM
16	sodium azide	natural abundance	0.05 %
17	beta-mercaptoethanol	natural abundance	2 mM
18	DSS	natural abundance	0.5 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
19	CdnL	[U-100% 13C; U-100% 15N]	1 mM
20	sodium phosphate	natural abundance	50 mM
21	sodium chloride	natural abundance	100 mM
22	sodium azide	natural abundance	0.05 %
23	beta-mercaptoethanol	natural abundance	2 mM
24	DSS	natural abundance	0.5 mM

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
25	CdnL	natural abundance	0.25 mM
26	sodium phosphate	natural abundance	50 mM
27	sodium chloride	natural abundance	100 mM
28	sodium azide	natural abundance	0.05 %
29	beta-mercaptoethanol	natural abundance	2 mM
30	DSS	natural abundance	0.1 mM

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
31	CdnL	natural abundance	0.25 mM
32	sodium phosphate	natural abundance	50 mM
33	sodium chloride	natural abundance	100 mM
34	sodium azide	natural abundance	0.05 %
35	beta-mercaptoethanol	natural abundance	2 mM
36	DSS	natural abundance	0.1 mM

Sample #7

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
37	CdnL	[U-100% 13C; U-100% 15N]	0.25 mM
38	sodium phosphate	natural abundance	50 mM
39	sodium chloride	natural abundance	100 mM
40	sodium azide	natural abundance	0.05 %
41	beta-mercaptoethanol	natural abundance	2 mM
42	DSS	natural abundance	0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LWJ, Strand ID: A Detail

Release date

2012-03-08

Citation

(1)H, (13)C and (15)N assignments of CdnL, an essential protein in Myxococcus xanthus
Mirassou, Y., Elias-Arnanz, M., Padmanabhan, S., Jimenez, M.
Biomol. NMR Assign. (2013), 7, 51-55, PubMed 22392343 , DOI 10.1007/s12104-012-9375-0 , Abstract

Related entities 1. CdnL, : 1 : 36 entities Detail

Experiments performed 21 experiments Detail

1 2D 1H-1H COSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CdnL	natural abundance	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	sodium azide	natural abundance	0.05 %
5	beta-mercaptoethanol	natural abundance	2 mM
6	DSS	natural abundance	0.5 mM

2 2D 1H-1H TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CdnL	natural abundance	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	sodium azide	natural abundance	0.05 %
5	beta-mercaptoethanol	natural abundance	2 mM
6	DSS	natural abundance	0.5 mM

3 2D 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	CdnL	natural abundance	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	sodium azide	natural abundance	0.05 %
5	beta-mercaptoethanol	natural abundance	2 mM
6	DSS	natural abundance	0.5 mM

4 2D 1H-1H COSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	CdnL	natural abundance	1 mM
8	sodium phosphate	natural abundance	50 mM
9	sodium chloride	natural abundance	100 mM
10	sodium azide	natural abundance	0.05 %
11	beta-mercaptoethanol	natural abundance	2 mM
12	DSS	natural abundance	0.5 mM

5 2D 1H-1H TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	CdnL	natural abundance	1 mM
8	sodium phosphate	natural abundance	50 mM
9	sodium chloride	natural abundance	100 mM
10	sodium azide	natural abundance	0.05 %
11	beta-mercaptoethanol	natural abundance	2 mM
12	DSS	natural abundance	0.5 mM

6 2D 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
7	CdnL	natural abundance	1 mM
8	sodium phosphate	natural abundance	50 mM
9	sodium chloride	natural abundance	100 mM
10	sodium azide	natural abundance	0.05 %
11	beta-mercaptoethanol	natural abundance	2 mM
12	DSS	natural abundance	0.5 mM

7 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
13	CdnL	[U-100% 13C; U-100% 15N]	1 mM
14	sodium phosphate	natural abundance	50 mM
15	sodium chloride	natural abundance	100 mM
16	sodium azide	natural abundance	0.05 %
17	beta-mercaptoethanol	natural abundance	2 mM
18	DSS	natural abundance	0.5 mM

8 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
13	CdnL	[U-100% 13C; U-100% 15N]	1 mM
14	sodium phosphate	natural abundance	50 mM
15	sodium chloride	natural abundance	100 mM
16	sodium azide	natural abundance	0.05 %
17	beta-mercaptoethanol	natural abundance	2 mM
18	DSS	natural abundance	0.5 mM

9 3D HNCA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
13	CdnL	[U-100% 13C; U-100% 15N]	1 mM
14	sodium phosphate	natural abundance	50 mM
15	sodium chloride	natural abundance	100 mM
16	sodium azide	natural abundance	0.05 %
17	beta-mercaptoethanol	natural abundance	2 mM
18	DSS	natural abundance	0.5 mM

10 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
13	CdnL	[U-100% 13C; U-100% 15N]	1 mM
14	sodium phosphate	natural abundance	50 mM
15	sodium chloride	natural abundance	100 mM
16	sodium azide	natural abundance	0.05 %
17	beta-mercaptoethanol	natural abundance	2 mM
18	DSS	natural abundance	0.5 mM

11 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
19	CdnL	[U-100% 13C; U-100% 15N]	1 mM
20	sodium phosphate	natural abundance	50 mM
21	sodium chloride	natural abundance	100 mM
22	sodium azide	natural abundance	0.05 %
23	beta-mercaptoethanol	natural abundance	2 mM
24	DSS	natural abundance	0.5 mM

12 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
19	CdnL	[U-100% 13C; U-100% 15N]	1 mM
20	sodium phosphate	natural abundance	50 mM
21	sodium chloride	natural abundance	100 mM
22	sodium azide	natural abundance	0.05 %
23	beta-mercaptoethanol	natural abundance	2 mM
24	DSS	natural abundance	0.5 mM

13 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
19	CdnL	[U-100% 13C; U-100% 15N]	1 mM
20	sodium phosphate	natural abundance	50 mM
21	sodium chloride	natural abundance	100 mM
22	sodium azide	natural abundance	0.05 %
23	beta-mercaptoethanol	natural abundance	2 mM
24	DSS	natural abundance	0.5 mM

14 2D 1H-1H COSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
25	CdnL	natural abundance	0.25 mM
26	sodium phosphate	natural abundance	50 mM
27	sodium chloride	natural abundance	100 mM
28	sodium azide	natural abundance	0.05 %
29	beta-mercaptoethanol	natural abundance	2 mM
30	DSS	natural abundance	0.1 mM

15 2D 1H-1H TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
25	CdnL	natural abundance	0.25 mM
26	sodium phosphate	natural abundance	50 mM
27	sodium chloride	natural abundance	100 mM
28	sodium azide	natural abundance	0.05 %
29	beta-mercaptoethanol	natural abundance	2 mM
30	DSS	natural abundance	0.1 mM

16 2D 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
25	CdnL	natural abundance	0.25 mM
26	sodium phosphate	natural abundance	50 mM
27	sodium chloride	natural abundance	100 mM
28	sodium azide	natural abundance	0.05 %
29	beta-mercaptoethanol	natural abundance	2 mM
30	DSS	natural abundance	0.1 mM

17 2D 1H-1H COSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
31	CdnL	natural abundance	0.25 mM
32	sodium phosphate	natural abundance	50 mM
33	sodium chloride	natural abundance	100 mM
34	sodium azide	natural abundance	0.05 %
35	beta-mercaptoethanol	natural abundance	2 mM
36	DSS	natural abundance	0.1 mM

18 2D 1H-1H TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
31	CdnL	natural abundance	0.25 mM
32	sodium phosphate	natural abundance	50 mM
33	sodium chloride	natural abundance	100 mM
34	sodium azide	natural abundance	0.05 %
35	beta-mercaptoethanol	natural abundance	2 mM
36	DSS	natural abundance	0.1 mM

19 2D 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
31	CdnL	natural abundance	0.25 mM
32	sodium phosphate	natural abundance	50 mM
33	sodium chloride	natural abundance	100 mM
34	sodium azide	natural abundance	0.05 %
35	beta-mercaptoethanol	natural abundance	2 mM
36	DSS	natural abundance	0.1 mM

20 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
37	CdnL	[U-100% 13C; U-100% 15N]	0.25 mM
38	sodium phosphate	natural abundance	50 mM
39	sodium chloride	natural abundance	100 mM
40	sodium azide	natural abundance	0.05 %
41	beta-mercaptoethanol	natural abundance	2 mM
42	DSS	natural abundance	0.5 mM

21 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
37	CdnL	[U-100% 13C; U-100% 15N]	0.25 mM
38	sodium phosphate	natural abundance	50 mM
39	sodium chloride	natural abundance	100 mM
40	sodium azide	natural abundance	0.05 %
41	beta-mercaptoethanol	natural abundance	2 mM
42	DSS	natural abundance	0.5 mM

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_18151_2lwj.nef
Input source #2: Coordindates	2lwj.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
GSHMQTSFKTGDKAVYPGQGVGEVMGIEHTEVAGQRQSFYVLRILENGMRIMIPINKVGSVGLREIISEEDVKQVYSILKEKDISVDSTTWNRRYREYME
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GSHMQTSFKTGDKAVYPGQGVGEVMGIEHTEVAGQRQSFYVLRILENGMRIMIPINKVGSVGLREIISEEDVKQVYSILKEKDISVDSTTWNRRYREYME

-------110-------120-------130-------140-------150-------160-------
KIKTGSVFEIAEVLRDLYLLKGDKDLSFGERKMLDTARSLLIKELSLAKDCSEDEIESDLKKIFNLA
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
KIKTGSVFEIAEVLRDLYLLKGDKDLSFGERKMLDTARSLLIKELSLAKDCSEDEIESDLKKIFNLA

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	167	0	0	100.0

Content subtype: combined_18151_2lwj.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1597		98.2 (chain: A, length: 167)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	Warning	4545 (1)	noe	96.4 (chain: A, length: 167)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_3	OK	289 (289)	.	97.0 (chain: A, length: 167)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 167, Sequence coverage: 98.2%
- Total number of assignments
  - ¹H chemical shifts: 892
  - ¹³C chemical shifts: 539
  - ¹⁵N chemical shifts: 166
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
5	GLN	CD	180.395
19	GLN	CD	177.956
35	GLN	CD	180.53
37	GLN	CD	180.005
47	ASN	CG	175.839
56	ASN	CG	177.218
74	GLN	CD	179.101
91	TRP	CD2	139.1
91	TRP	CE2	169.05
92	ASN	CG	176.439
165	ASN	CG	178.303

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1036	892	86.1
¹³C chemical shifts	770	528	68.6
¹⁵N chemical shifts	185	166	89.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	345	318	92.2
¹³C chemical shifts	334	300	89.8
¹⁵N chemical shifts	165	152	92.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	691	574	83.1
¹³C chemical shifts	436	228	52.3
¹⁵N chemical shifts	20	14	70.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	103	95	92.2
¹³C chemical shifts	103	88	85.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	59	56	94.9
¹³C chemical shifts	58	0	0.0
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 167, Sequence coverage: 96.4%
- Total number of restraints: 4544
- Weight of restraints: 1.0 (4544)
- Potential type of restraints: upper-bound-parabolic (4544)
- Range of distance target values: 2.28, 5.5 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 167, Sequence coverage: 97.0%
- Total number of restraints: 289
- Total number of restraints per polymer type: 289
- Weight of restraints: 1.0 (289)
- Potential type of restraints: square-well-parabolic (289)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords CarD_TRCF, CdnL, Myxococcus xanthus, PF02559, RNA polymerase interacting domain

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Found 1 Document (1.139 seconds)

BMRB: 18151

Sample #1

Sample #2

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Sample #4

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Sample #7

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. CdnL, : 1 : 36 entities Detail

Experiments performed 21 experiments Detail

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