Solution NMR Structure of the mitochondrial inner membrane domain (residues 164-251), FtsH_ext, from the paraplegin-like protein AFGL32 from Homo sapiens, Northeast Structural Genomics Consortium Target HR6741A
MGHHHHHHSH MKRSGREITW KDFVNNYLSK GVVDRLEVVN KRFVRVTFTP GKTPVDGQYV WFNIGSVDTF ERNLETLQQE LGIEGENRVP VVYIAESDG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.5 % (1063 of 1174) | 90.8 % (553 of 609) | 89.7 % (411 of 458) | 92.5 % (99 of 107) |
Backbone | 90.5 % (532 of 588) | 91.2 % (186 of 204) | 89.6 % (258 of 288) | 91.7 % (88 of 96) |
Sidechain | 90.7 % (613 of 676) | 90.6 % (367 of 405) | 90.4 % (235 of 260) | 100.0 % (11 of 11) |
Aromatic | 77.8 % (98 of 126) | 77.8 % (49 of 63) | 77.0 % (47 of 61) | 100.0 % (2 of 2) |
Methyl | 100.0 % (102 of 102) | 100.0 % (51 of 51) | 100.0 % (51 of 51) |
1. HR6741A
MGHHHHHHSH MKRSGREITW KDFVNNYLSK GVVDRLEVVN KRFVRVTFTP GKTPVDGQYV WFNIGSVDTF ERNLETLQQE LGIEGENRVP VVYIAESDGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | MES | natural abundance | 20 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | DSS | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM NC5 HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | HR6741A | NC5 | 1.0 mM | |
11 | sodium chloride | natural abundance | 200 mM | |
12 | MES | natural abundance | 20 mM | |
13 | calcium chloride | natural abundance | 5 mM | |
14 | DTT | natural abundance | 10 mM | |
15 | DSS | natural abundance | 10 uM | |
16 | sodium azide | natural abundance | 0.02 % | |
17 | H2O | natural abundance | 90 % | |
18 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
20 | sodium chloride | natural abundance | 200 mM | |
21 | MES | natural abundance | 20 mM | |
22 | calcium chloride | natural abundance | 5 mM | |
23 | DTT | natural abundance | 10 mM | |
24 | DSS | natural abundance | 10 uM | |
25 | sodium azide | natural abundance | 0.02 % | |
26 | D2O | natural abundance | 100 % |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | na | indirect | 0.2514495 |
1H | DSS | methyl protons | 0.0 ppm | internal | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | na | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | na | indirect | 0.2514495 |
1H | DSS | methyl protons | 0.0 ppm | internal | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | na | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | na | indirect | 0.2514495 |
1H | DSS | methyl protons | 0.0 ppm | internal | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | na | indirect | 0.1013291 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
13C | DSS | methyl protons | 0.0 ppm | na | indirect | 0.2514495 |
1H | DSS | methyl protons | 0.0 ppm | internal | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | na | indirect | 0.1013291 |
Bruker AvanceIII - 850 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | MES | natural abundance | 20 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | DSS | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Bruker AvanceIII - 850 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | MES | natural abundance | 20 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | DSS | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | MES | natural abundance | 20 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | DSS | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | MES | natural abundance | 20 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | DSS | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | MES | natural abundance | 20 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | DSS | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Bruker AvanceIII - 600 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | MES | natural abundance | 20 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | DSS | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Bruker AvanceIII - 850 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | MES | natural abundance | 20 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | DSS | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Bruker AvanceIII - 850 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | MES | natural abundance | 20 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | DSS | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Bruker AvanceIII - 600 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM NC5 HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | HR6741A | NC5 | 1.0 mM | |
11 | sodium chloride | natural abundance | 200 mM | |
12 | MES | natural abundance | 20 mM | |
13 | calcium chloride | natural abundance | 5 mM | |
14 | DTT | natural abundance | 10 mM | |
15 | DSS | natural abundance | 10 uM | |
16 | sodium azide | natural abundance | 0.02 % | |
17 | H2O | natural abundance | 90 % | |
18 | D2O | natural abundance | 10 % |
Bruker AvanceIII - 600 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM NC5 HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | HR6741A | NC5 | 1.0 mM | |
11 | sodium chloride | natural abundance | 200 mM | |
12 | MES | natural abundance | 20 mM | |
13 | calcium chloride | natural abundance | 5 mM | |
14 | DTT | natural abundance | 10 mM | |
15 | DSS | natural abundance | 10 uM | |
16 | sodium azide | natural abundance | 0.02 % | |
17 | H2O | natural abundance | 90 % | |
18 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | MES | natural abundance | 20 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | DSS | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | MES | natural abundance | 20 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | DSS | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Bruker AvanceIII - 600 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | MES | natural abundance | 20 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | DSS | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | MES | natural abundance | 20 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | DSS | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | MES | natural abundance | 20 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | DSS | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz at MU
State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
2 | sodium chloride | natural abundance | 200 mM | |
3 | MES | natural abundance | 20 mM | |
4 | calcium chloride | natural abundance | 5 mM | |
5 | DTT | natural abundance | 10 mM | |
6 | DSS | natural abundance | 10 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Varian INOVA - 600 MHz at MU
State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
20 | sodium chloride | natural abundance | 200 mM | |
21 | MES | natural abundance | 20 mM | |
22 | calcium chloride | natural abundance | 5 mM | |
23 | DTT | natural abundance | 10 mM | |
24 | DSS | natural abundance | 10 uM | |
25 | sodium azide | natural abundance | 0.02 % | |
26 | D2O | natural abundance | 100 % |
Varian INOVA - 600 MHz at MU
State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
20 | sodium chloride | natural abundance | 200 mM | |
21 | MES | natural abundance | 20 mM | |
22 | calcium chloride | natural abundance | 5 mM | |
23 | DTT | natural abundance | 10 mM | |
24 | DSS | natural abundance | 10 uM | |
25 | sodium azide | natural abundance | 0.02 % | |
26 | D2O | natural abundance | 100 % |
Varian INOVA - 600 MHz at MU
State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HR6741A, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | HR6741A | [U-100% 13C; U-100% 15N] | 0.7 mM | |
20 | sodium chloride | natural abundance | 200 mM | |
21 | MES | natural abundance | 20 mM | |
22 | calcium chloride | natural abundance | 5 mM | |
23 | DTT | natural abundance | 10 mM | |
24 | DSS | natural abundance | 10 uM | |
25 | sodium azide | natural abundance | 0.02 % | |
26 | D2O | natural abundance | 100 % |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints | combined_18156_2lna.nef |
Input source #2: Coordindates | 2lna.cif |
Diamagnetism of the molecular assembly | True (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | None |
Whether the assembly has a other bond | None |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Warning |
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneSequence alignments
--------10--------20--------30--------40--------50--------60--------70--------80--------90--------- MGHHHHHHSHMKRSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQYVWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDG ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| MGHHHHHHSHMKRSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQYVWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDG
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 99 | 0 | 0 | 100.0 |
Content subtype: combined_18156_2lna.nef
Assigned chemical shifts
--------10--------20--------30--------40--------50--------60--------70--------80--------90--------- MGHHHHHHSHMKRSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQYVWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDG | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| .G.......HMKRSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQYVWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDG
Comp_index_ID | Comp_ID | Atom_ID | CS value (ppm) |
---|---|---|---|
19 | THR | HG1 | 5.17 |
25 | ASN | CG | 175.9 |
26 | ASN | CG | 176.8 |
40 | ASN | CG | 178.2 |
58 | GLN | CD | 180.2 |
63 | ASN | CG | 175.5 |
73 | ASN | CG | 175.8 |
78 | GLN | CD | 177.9 |
79 | GLN | CD | 180.2 |
87 | ASN | CG | 177.1 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 609 | 554 | 91.0 |
13C chemical shifts | 458 | 411 | 89.7 |
15N chemical shifts | 114 | 101 | 88.6 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 204 | 186 | 91.2 |
13C chemical shifts | 198 | 177 | 89.4 |
15N chemical shifts | 96 | 86 | 89.6 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 405 | 368 | 90.9 |
13C chemical shifts | 260 | 234 | 90.0 |
15N chemical shifts | 18 | 15 | 83.3 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 53 | 51 | 96.2 |
13C chemical shifts | 53 | 51 | 96.2 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 63 | 48 | 76.2 |
13C chemical shifts | 61 | 46 | 75.4 |
15N chemical shifts | 2 | 2 | 100.0 |
Distance restraints
--------10--------20--------30--------40--------50--------60--------70--------80--------90--------- MGHHHHHHSHMKRSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQYVWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDG ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ..........MKRSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQYVWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDG
--------10--------20--------30--------40--------50--------60--------70--------80--------90--------- MGHHHHHHSHMKRSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQYVWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDG | ||||||||| || || ||||||| ||||| | ||| |||||||||||||||| | | | | | ...............R.ITWKDFVNN.LS.GV.DRLEVVN..FVRVT.T..........VWF...SVDTFERNLETLQQEL..E..N..P.V.I --------10--------20--------30--------40--------50--------60--------70--------80--------90----
Dihedral angle restraints
--------10--------20--------30--------40--------50--------60--------70--------80--------90--------- MGHHHHHHSHMKRSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQYVWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDG |||||||||||||||||||||||||||||||||||| ||||| |||||| ||||||||||||||||||||||||||||||| ..............GREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTP.KTPVD.QYVWFN.GSVDTFERNLETLQQELGIEGENRVPVVYIA --------10--------20--------30--------40--------50--------60--------70--------80--------90-----