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BMRB: 18158

2LNB

Solution NMR structure of N-terminal domain (6-74) of human ZBP1 protein, Northeast Structural Genomics Consortium Target HR8174A.

Authors

Wang, D., Yang, Y., Ramelot, T.A., Hamilton, K., Kohan, E., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Kennedy, M.A.

Assembly

N-terminal domain (6-74) of human ZBP1 protein

Entity

1. N-terminal domain (6-74) of human ZBP1 protein (polymer, Thiol state: all free), 80 monomers, 9023.260 Da Detail

MGHHHHHHSH MADPGREGHL EQRILQVLTE AGSPVKLAQL VKECQAPKRE LNQVLYRMKK ELKVSLTSPA TWCLGGTDPE

Formula weight

9023.26 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.3 %, Completeness: 88.6 %, Completeness (bb): 89.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.6 % (825 of 931)	89.0 % (435 of 489)	87.8 % (316 of 360)	90.2 % (74 of 82)
Backbone	89.1 % (419 of 470)	88.8 % (143 of 161)	89.3 % (209 of 234)	89.3 % (67 of 75)
Sidechain	88.4 % (473 of 535)	89.0 % (292 of 328)	87.0 % (174 of 200)	100.0 % (7 of 7)
Aromatic	42.3 % (22 of 52)	46.2 % (12 of 26)	36.0 % (9 of 25)	100.0 % (1 of 1)
Methyl	100.0 % (82 of 82)	100.0 % (41 of 41)	100.0 % (41 of 41)

1. N-terminal domain (6-74) of human ZBP1 protein

MGHHHHHHSH MADPGREGHL EQRILQVLTE AGSPVKLAQL VKECQAPKRE LNQVLYRMKK ELKVSLTSPA TWCLGGTDPE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	N-terminal domain (6-74) of human ZBP1 protein	[U-100% 13C; U-100% 15N]	1.0 (±0.1) mM
2	MES	natural abundance	20 (±1.0) mM
3	sodium chloride	natural abundance	100 (±5.0) mM
4	DTT	natural abundance	10 (±0.5) mM
5	sodium azide	natural abundance	0.02 (±0.001) %
6	calcium chloride	natural abundance	5 (±0.25) mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	N-terminal domain (6-74) of human ZBP1 protein	[U-5% 13C; U-100% 15N]	1.00 (±0.1) mM
8	MES	natural abundance	20 (±1.0) mM
9	sodium chloride	natural abundance	100 (±5.0) mM
10	DTT	natural abundance	10 (±0.5) mM
11	sodium azide	natural abundance	0.02 (±0.001) %
12	calcium chloride	natural abundance	5 (±0.25) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.61 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.61 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.33 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LNB, Strand ID: A Detail

Release date

2012-01-25

Citation

Solution NMR structure of N-terminal domain (6-74) of human ZBP1 protein, Northeast Structural Genomics Consortium Target HR8174A
Yang, Y., Ramelot, T.A., Hamilton, K., Kohan, E., Wang, D., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Kennedy, M.A.
Not known

Related entities 1. N-terminal domain (6-74) of human ZBP1 protein, : 1 : 2 : 4 entities Detail

Interaction partners 1. N-terminal domain (6-74) of human ZBP1 protein, : 5 interactors Detail

More details for 5 interactors identified by 2 citations

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC