BMRB: 18159

Solution NMR structure of the C-terminal extension of two bacterial light, oxygen, voltage (LOV) photoreceptor proteins from Pseudomonas putida

Authors

Rani, R., Lecher, J., Hartmann, R., Jaeger, K., Willbold, D., Krauss, U.

Assembly

SB1Jalpha

Entity

1. SB1Jalpha (polymer, Thiol state: not present), 24 monomers, 2775.083 Da Detail

XSRQVELERE LAELRARPKP DERA

Formula weight

2775.083 Da

Source organism

Pseudomonas putida

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.8 %, Completeness: 100.0 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	100.0 % (150 of 150)	100.0 % (150 of 150)
Backbone	100.0 % (44 of 44)	100.0 % (44 of 44)
Sidechain	100.0 % (106 of 106)	100.0 % (106 of 106)
Methyl	100.0 % (11 of 11)	100.0 % (11 of 11)

1. AceSB1JalphaPep

XSRQVELERE LAELRARPKP DERA

Show sample condition

Sample

Solvent system trifluoroethanol/water, Pressure 1.000 atm, Temperature 298.000 K, pH 6.300, Details 10%TFE

#	Name	Isotope labeling	Type	Concentration
1	AceSB1JalphaPep	natural abundance		1.0 mM
2	deuteriumoxide	natural abundance		10.0 %
3	sodium phosphate	natural abundance		100.0 mM
4	sodium chloride	natural abundance		50.0 mM
5	2,2,2-Trifluoroethanol	natural abundance		10.0 %
6	H2O	natural abundance		80.0 %

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Calculated from 10 models in PDB: 2YOM, Strand ID: A Detail

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Release date

2013-07-07

Citation

Conservation of dark recovery kinetic parameters and structural features in the pseudomonadaceae "short" light, oxygen, voltage (LOV) protein family: implications for the design of LOV-based optogenetic tools
Rani, R., Jentzsch, K., Lecher, J., Hartmann, R., Willbold, D., Jaeger, K., Krauss, U.
Biochemistry (2013), 52, 4460-4473, PubMed 23746326 , DOI 10.1021/bi400311r , Abstract

Related entities 1. SB1Jalpha, : 1 : 3 : 1 entities Detail

Experiments performed 1 experiments Detail