BMRB: 18160

Solution NMR structure of the C-terminal extension of two bacterial light, oxygen, voltage (LOV) photoreceptor proteins from Pseudomonas putida

Authors

Rani, R., Lecher, J., Hartmann, R., Krauss, U., Jaeger, K., Willbold, D.

Assembly

SB2Jalpha

Entity

1. SB2Jalpha (polymer, Thiol state: not present), 30 monomers, 3405.729 Da Detail

XTAQVFAEER VRELEAEVAE LRRQQGQAKH

Formula weight

3405.729 Da

Source organism

Pseudomonas putida

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 99.4 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	99.4 % (179 of 180)	99.4 % (179 of 180)
Backbone	100.0 % (59 of 59)	100.0 % (59 of 59)
Sidechain	99.2 % (120 of 121)	99.2 % (120 of 121)
Aromatic	85.7 % (6 of 7)	85.7 % (6 of 7)
Methyl	100.0 % (16 of 16)	100.0 % (16 of 16)

1. AceSB2Pep

XTAQVFAEER VRELEAEVAE LRRQQGQAKH

Show sample condition

Sample

Solvent system trifluoroethanol/water, Pressure 1.000 atm, Temperature 298.000 K, pH 6.300, Details 25%TFE

#	Name	Isotope labeling	Type	Concentration
1	AceSB2Pep	natural abundance		1.0 mM
2	sodium phosphate	natural abundance		100.0 mM
3	sodium chloride	natural abundance		50.0 mM
4	2,2,2-Trifluoroethanol	natural abundance		25.0 %
5	H2O	natural abundance		65.0 %
6	deuterium oxide	natural abundance		10.0 %

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Protein Blocks Logo

Calculated from 10 models in PDB: 2YON, Strand ID: A Detail

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Release date

2013-07-07

Citation

Conservation of dark recovery kinetic parameters and structural features in the pseudomonadaceae "short" light, oxygen, voltage (LOV) protein family: implications for the design of LOV-based optogenetic tools
Rani, R., Jentzsch, K., Lecher, J., Hartmann, R., Willbold, D., Jaeger, K., Krauss, U.
Biochemistry (2013), 52, 4460-4473, PubMed 23746326 , DOI 10.1021/bi400311r , Abstract

Related entities 1. SB2Jalpha, : 1 : 3 entities Detail

Experiments performed 1 experiments Detail