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BMRB: 18167

2LNJ

Solution Structure of Cyanobacterial PsbP (CyanoP) from Synechocystis sp. PCC 6803

Authors

Jackson, S.A., Hinds, M.G., Eaton-Rye, J.J.

Assembly

PsbP

Entity

1. PsbP (polymer), 170 monomers, 18726.42 Da Detail

GPLGSCGGVG IASLQRYSDT KDGYEFLYPN GWIGVDVKGA SPGVDVVFRD LIERDENLSV IISEIPSDKT LTDLGTATDV GYRFMKTVND ASQGDRQAEL INAEARDEDG QVYYTLEYRV LVGDNVERHD LASVTTNRGK LITFDLSTAE DRWDTVKSLF DTVASSFHVY

Formula weight

18726.42 Da

Source organism

Synechocystis sp.

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 90.6 %, Completeness (bb): 98.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.6 % (1710 of 1887)	92.3 % (890 of 964)	86.3 % (643 of 745)	99.4 % (177 of 178)
Backbone	98.7 % (999 of 1012)	98.0 % (345 of 352)	99.0 % (489 of 494)	99.4 % (165 of 166)
Sidechain	83.9 % (863 of 1029)	89.1 % (545 of 612)	75.6 % (306 of 405)	100.0 % (12 of 12)
Aromatic	48.7 % (76 of 156)	89.7 % (70 of 78)	5.3 % (4 of 76)	100.0 % (2 of 2)
Methyl	96.5 % (195 of 202)	97.0 % (98 of 101)	96.0 % (97 of 101)

1. entity

GPLGSCGGVG IASLQRYSDT KDGYEFLYPN GWIGVDVKGA SPGVDVVFRD LIERDENLSV IISEIPSDKT LTDLGTATDV GYRFMKTVND ASQGDRQAEL INAEARDEDG QVYYTLEYRV LVGDNVERHD LASVTTNRGK LITFDLSTAE DRWDTVKSLF DTVASSFHVY

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298.15 K, pH 6.7

#	Name	Isotope labeling	Concentration
1	PsbP	[U-100% 15N]	0.4 mM
2	TCEP	natural abundance	5 mM
3	sodium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	10 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298.15 K, pH 6.7

#	Name	Isotope labeling	Concentration
7	PsbP	[U-100% 13C; U-100% 15N]	0.4 mM
8	TCEP	natural abundance	5 mM
9	sodium phosphate	natural abundance	25 mM
10	sodium chloride	natural abundance	10 mM
11	H2O	natural abundance	95 %
12	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.32 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LNJ, Strand ID: A Detail

Release date

2012-03-22

Citation

Solution structure of CyanoP from Synechocystis sp. PCC 6803: new insights on the structural basis for functional specialization amongst PsbP family proteins
Jackson, S.A., Hinds, M.G., Eaton-Rye, J.J.
Biochim. Biophys. Acta (2012), 1817, 1331-1338, PubMed 22414666 , DOI 10.1016/j.bbabio.2012.02.032 , Abstract

Related entities 1. PsbP, : 1 : 64 entities Detail

Experiments performed 9 experiments Detail

1 3D 1H-13C NOESY