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BMRB: 18180

2LNU

Solution NMR Structure of the uncharacterized protein from gene locus rrnAC0354 of Haloarcula marismortui. Northeast Structural Genomics Consortium Target HmR11.

Authors

Rossi, P., Liu, G., Lange, O.F., Lee, H., Janjua, H., Ciccosanti, C., Wang, H., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T.

Assembly

HmR11

Entity

1. HmR11 (polymer, Thiol state: all free), 185 monomers, 21378.59 Da Detail

MDEDTEDWET QLQANRDEKD RFFSEHRQSP IPPEERDDFD GLSYFDPDPD YRVEATVTVH ETPESVDLET SDDRTVRYLH VATLSFDLDG ESRDLHAFRQ AADESRTLFV PFRDKTTGQQ SYDGGRYMEL EPDRDLSDGD EITLDFNLAY SPFCAYSDTF SCPLPPESNW LETAVTAGER TDLEH

Formula weight

21378.59 Da

Source organism

Haloarcula marismortui

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.7 %, Completeness (bb): 98.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.7 % (2040 of 2087)	97.6 % (1055 of 1081)	97.9 % (805 of 822)	97.8 % (180 of 184)
Backbone	98.3 % (1068 of 1086)	98.1 % (358 of 365)	98.4 % (539 of 548)	98.8 % (171 of 173)
Sidechain	97.4 % (1148 of 1179)	97.3 % (697 of 716)	97.8 % (442 of 452)	81.8 % (9 of 11)
Aromatic	96.2 % (202 of 210)	97.1 % (102 of 105)	95.1 % (98 of 103)	100.0 % (2 of 2)
Methyl	97.4 % (148 of 152)	97.4 % (74 of 76)	97.4 % (74 of 76)

1. HmR11

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.7 mM [U-100% 13C; U-100% 15N] HmR11, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	HmR11-1	[U-100% 13C; U-100% 15N]	0.7 mM
2	NaN3-2	natural abundance	0.02 %
3	DTT-3	natural abundance	10 mM
4	CaCL2-4	natural abundance	5 mM
5	NaCL-5	natural abundance	100 mM
6	Proteinase Inhibitors-6	natural abundance	1 na
7	MES pH 6.5-7	natural abundance	20 mM
8	D2O-8	natural abundance	10 %
9	DSS-9	natural abundance	50 uM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.2 mM [5% 13C; U-100% 15N] HmR11, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
10	HmR11-1	[U-5% 13C; U-100% 15N]	1.2 mM
11	NaN3-2	natural abundance	0.02 %
12	DTT-3	natural abundance	10 mM
13	CaCL2-4	natural abundance	5 mM
14	NaCL-5	natural abundance	100 mM
15	Proteinase Inhibitors-6	natural abundance	1 na
16	MES pH 6.5-7	natural abundance	20 mM
17	D2O-8	natural abundance	10 %
18	DSS-9	natural abundance	50 uM

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.75 mM [5% 13C; U-100% 15N] HmR11, 95% H2O/5% D2O split and aligned in PEG & Phage

#	Name	Isotope labeling	Concentration
19	HmR11	[U-5% 13C; U-100% 15N]	0.75 mM
20	NaN3-2	natural abundance	0.02 %
21	DTT-3	natural abundance	10 mM
22	CaCL2-4	natural abundance	5 mM
23	NaCL-5	natural abundance	100 mM
24	Proteinase Inhibitors-6	natural abundance	1 na
25	MES pH 6.5-7	natural abundance	20 mM
26	D2O-8	natural abundance	10 %
27	DSS-9	natural abundance	50 uM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LNU, Strand ID: A Detail

Release date

2012-01-11

Related entities 1. HmR11, : 1 : 3 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC