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BMRB: 18183

2LNX

Identification and structural basis for a novel interaction between Vav2 and Arap3

Authors

Wu, B., Zhang, J., Wu, J., Shi, Y.

Assembly

Vav2

Entity

1. Vav2 (polymer, Thiol state: all free), 113 monomers, 13458.01 Da Detail

SRPPSREIDY TAYPWFAGNM ERQQTDNLLK SHASGTYLIR ERPAEAERFA ISIKFNDEVK HIKVVEKDNW IHITEAKKFD SLLELVEYYQ CHSLKESFKQ LDTTLKYPYK SRE

Formula weight

13458.01 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.8 %, Completeness (bb): 95.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.8 % (1256 of 1398)	95.4 % (701 of 735)	80.7 % (440 of 545)	97.5 % (115 of 118)
Backbone	95.5 % (638 of 668)	96.4 % (215 of 223)	94.1 % (317 of 337)	98.1 % (106 of 108)
Sidechain	85.9 % (722 of 841)	94.9 % (486 of 512)	71.2 % (227 of 319)	90.0 % (9 of 10)
Aromatic	50.0 % (73 of 146)	89.0 % (65 of 73)	9.9 % (7 of 71)	50.0 % (1 of 2)
Methyl	98.1 % (104 of 106)	100.0 % (53 of 53)	96.2 % (51 of 53)

1. entity

SRPPSREIDY TAYPWFAGNM ERQQTDNLLK SHASGTYLIR ERPAEAERFA ISIKFNDEVK HIKVVEKDNW IHITEAKKFD SLLELVEYYQ CHSLKESFKQ LDTTLKYPYK SRE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Temperature 293 K, pH 6.2

#	Name	Isotope labeling	Concentration
1	entity	[U-13C; U-15N]	1 mM
2	DTT	natural abundance	5 mM
3	sodium chloride	natural abundance	75 mM
4	potassium phosphate	natural abundance	5 mM
5	sodium phosphate	natural abundance	20 mM
6	EDTA	natural abundance	1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Temperature 293 K, pH 6.2

#	Name	Isotope labeling	Concentration
9	entity	[U-13C; U-15N]	1 mM
10	sodium chloride	natural abundance	75 mM
11	DTT	natural abundance	5 mM
12	potassium phosphate	natural abundance	5 mM
13	sodium phosphate	natural abundance	20 mM
14	EDTA	natural abundance	1 mM
15	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.63 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LNX, Strand ID: A Detail

Release date

2012-01-18

Citation

Identification and structural basis for a novel interaction between Vav2 and Arap3
Wu, B., Wang, F., Zhang, J., Zhang, Z., Qin, L., Peng, J., Li, F., Liu, J., Lu, G., Gong, Q., Yao, X., Wu, J., Shi, Y.
J. Struct. Biol. (2012), 180, 84-95, PubMed 22750419 , DOI 10.1016/j.jsb.2012.06.011 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 18182 released on 2012-11-18
Title Identification and structural basis for a novel interaction between Vav2 and Arap3

Related entities 1. Vav2, : 1 : 4 : 3 : 232 entities Detail

Interaction partners 1. Vav2, : 68 interactors Detail

More details for 68 interactors identified by 14 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC