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BMRB: 18200

2MLK

Resonance assignments for C-terminal DNA-binding domain of RstA protein from Klebsiella pneumonia

Authors

Chen, S., Fang, P., Cheng, Y., Chang, C., Yu, T., Huang, T.

Assembly

RstA C

Entity

1. RstA C (polymer, Thiol state: not present), 119 monomers, 13436.16 Da Detail

MHHHHHHAMG TLTPHKTISF GSLTIDPVNR QVLLGGENVA LSTADFDLLW ELATHAGQIM DRDALLKNLR GVTYDGMDRS VDVAISRLRK KLLDNATEPY RIKTVRNKGY LFAPHAWDN

Formula weight

13436.16 Da

Source organism

Klebsiella pneumoniae

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.2 %, Completeness: 75.3 %, Completeness (bb): 86.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.3 % (1035 of 1375)	75.6 % (535 of 708)	73.1 % (396 of 542)	83.2 % (104 of 125)
Backbone	86.5 % (611 of 706)	86.8 % (210 of 242)	85.4 % (298 of 349)	89.6 % (103 of 115)
Sidechain	66.5 % (519 of 780)	69.7 % (325 of 466)	63.5 % (193 of 304)	10.0 % (1 of 10)
Aromatic	24.6 % (28 of 114)	33.3 % (19 of 57)	16.4 % (9 of 55)	0.0 % (0 of 2)
Methyl	84.2 % (123 of 146)	95.9 % (70 of 73)	72.6 % (53 of 73)

1. RstA C

MHHHHHHAMG TLTPHKTISF GSLTIDPVNR QVLLGGENVA LSTADFDLLW ELATHAGQIM DRDALLKNLR GVTYDGMDRS VDVAISRLRK KLLDNATEPY RIKTVRNKGY LFAPHAWDN

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Type	Concentration
1	RstA_C	[U-13C; U-15N]		0.1-1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.29 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MLK, Strand ID: A Detail

Release date

2012-05-07

Citation

(1)H, (13)C and (15)N resonance assignments of the C-terminal DNA-binding domain of RstA protein from Klebsiella pneumoniae
Chen, S., Chang, C., Fan, P., Cheng, Y., Yu, T., Huang, T.
Biomol. NMR Assign. (2013), 7, 85-88, PubMed 22481468 , DOI 10.1007/s12104-012-9383-0 , Abstract

Related entities 1. RstA C, : 1 : 1 : 154 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC