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BMRB: 18229

2LOX

NMR structure of the complex between the PH domain of the Tfb1 subunit from TFIIH and Rad2

Authors

Lafrance-Vanasse, J., Legault, P., Omichinski, J.

Assembly

complex between the PH domain of the Tfb1 subunit from TFIIH and Rad2

Entity

1. Tfb1 (polymer), 119 monomers, 13228.85 Da Detail

PSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDADGNSS

2. Rad2 (polymer), 52 monomers, 6007.186 Da Detail

GSEILERESE KESSNDENKD DDLEVLSEEL FEDVPTKSQI SKEAEDNDSR KY

Total weight

19236.035 Da

Max. entity weight

13228.85 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.9 %, Completeness: 90.5 %, Completeness (bb): 92.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.5 % (1773 of 1959)	88.7 % (914 of 1030)	92.2 % (690 of 748)	93.4 % (169 of 181)
Backbone	92.4 % (937 of 1014)	89.8 % (308 of 343)	94.1 % (476 of 506)	92.7 % (153 of 165)
Sidechain	89.6 % (994 of 1109)	88.2 % (606 of 687)	91.6 % (372 of 406)	100.0 % (16 of 16)
Aromatic	70.0 % (56 of 80)	70.0 % (28 of 40)	69.2 % (27 of 39)	100.0 % (1 of 1)
Methyl	95.6 % (153 of 160)	97.5 % (78 of 80)	93.8 % (75 of 80)

1. Tfb1

PSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDADGNSS

2. Rad2

GSEILERESE KESSNDENKD DDLEVLSEEL FEDVPTKSQI SKEAEDNDSR KY

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Tfb1	[U-100% 13C; U-100% 15N]	1 mM
2	Rad2	natural abundance	1.25 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	1 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Tfb1	[U-100% 13C; U-100% 15N]	1 mM
9	Rad2	natural abundance	1.25 mM
10	sodium phosphate	natural abundance	20 mM
11	EDTA	natural abundance	1 mM
12	DTT	natural abundance	1 mM
13	D2O	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Tfb1	[U-100% 15N]	1 mM
15	Rad2	natural abundance	1.25 mM
16	sodium phosphate	natural abundance	20 mM
17	EDTA	natural abundance	1 mM
18	DTT	natural abundance	1 mM
19	H2O	natural abundance	90 %
20	D2O	natural abundance	10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
21	Tfb1	natural abundance	1.25 mM
22	Rad2	[U-100% 13C; U-100% 15N]	1 mM
23	sodium phosphate	natural abundance	20 mM
24	EDTA	natural abundance	1 mM
25	DTT	natural abundance	1 mM
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
28	Tfb1	natural abundance	1.25 mM
29	Rad2	[U-100% 13C; U-100% 15N]	1 mM
30	sodium phosphate	natural abundance	20 mM
31	EDTA	natural abundance	1 mM
32	DTT	natural abundance	1 mM
33	D2O	natural abundance	100 %

Sample #6

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
34	Tfb1	natural abundance	1.25 mM
35	Rad2	[U-100% 15N]	1 mM
36	sodium phosphate	natural abundance	20 mM
37	EDTA	natural abundance	1 mM
38	DTT	natural abundance	1 mM
39	H2O	natural abundance	90 %
40	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LOX, Strand ID: A, B Detail

Release date

2012-03-01

Citation

Structural and functional characterization of interactions involving the Tfb1 subunit of TFIIH and the NER factor Rad2
Lafrance-Vanasse, J., Arseneault, G., Cappadocia, L., Chen, H., Legault, P., Omichinski, J.G.
Nucleic Acids Res. (2012), 40, 5739-5750, PubMed 22373916 , DOI 10.1093/nar/gks194 , Abstract

Related entities 1. Tfb1, : 2 : 4 entities Detail

Related entities 2. Rad2, : 1 : 2 entities Detail

Interaction partners 1. Tfb1, : 49 interactors Detail

More details for 49 interactors identified by 12 citations

Interaction partners 2. Rad2, : 8 interactors Detail

More details for 8 interactors identified by 3 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Tfb1	[U-100% 15N]	1 mM
15	Rad2	natural abundance	1.25 mM
16	sodium phosphate	natural abundance	20 mM
17	EDTA	natural abundance	1 mM
18	DTT	natural abundance	1 mM
19	H2O	natural abundance	90 %
20	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Tfb1	[U-100% 13C; U-100% 15N]	1 mM
9	Rad2	natural abundance	1.25 mM
10	sodium phosphate	natural abundance	20 mM
11	EDTA	natural abundance	1 mM
12	DTT	natural abundance	1 mM
13	D2O	natural abundance	100 %

3 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Tfb1	[U-100% 13C; U-100% 15N]	1 mM
2	Rad2	natural abundance	1.25 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	1 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

4 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Tfb1	[U-100% 13C; U-100% 15N]	1 mM
2	Rad2	natural abundance	1.25 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	1 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

5 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Tfb1	[U-100% 13C; U-100% 15N]	1 mM
2	Rad2	natural abundance	1.25 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	1 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

6 3D H(CCO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Tfb1	[U-100% 13C; U-100% 15N]	1 mM
2	Rad2	natural abundance	1.25 mM
3	sodium phosphate	natural abundance	20 mM
4	EDTA	natural abundance	1 mM
5	DTT	natural abundance	1 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

7 3D HCCH-COSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Tfb1	[U-100% 13C; U-100% 15N]	1 mM
9	Rad2	natural abundance	1.25 mM
10	sodium phosphate	natural abundance	20 mM
11	EDTA	natural abundance	1 mM
12	DTT	natural abundance	1 mM
13	D2O	natural abundance	100 %

8 3D 1H-15N NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
14	Tfb1	[U-100% 15N]	1 mM
15	Rad2	natural abundance	1.25 mM
16	sodium phosphate	natural abundance	20 mM
17	EDTA	natural abundance	1 mM
18	DTT	natural abundance	1 mM
19	H2O	natural abundance	90 %
20	D2O	natural abundance	10 %

9 3D 1H-13C NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Tfb1	[U-100% 13C; U-100% 15N]	1 mM
9	Rad2	natural abundance	1.25 mM
10	sodium phosphate	natural abundance	20 mM
11	EDTA	natural abundance	1 mM
12	DTT	natural abundance	1 mM
13	D2O	natural abundance	100 %

10 2D 1H-15N HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
34	Tfb1	natural abundance	1.25 mM
35	Rad2	[U-100% 15N]	1 mM
36	sodium phosphate	natural abundance	20 mM
37	EDTA	natural abundance	1 mM
38	DTT	natural abundance	1 mM
39	H2O	natural abundance	90 %
40	D2O	natural abundance	10 %

11 2D 1H-13C HSQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
28	Tfb1	natural abundance	1.25 mM
29	Rad2	[U-100% 13C; U-100% 15N]	1 mM
30	sodium phosphate	natural abundance	20 mM
31	EDTA	natural abundance	1 mM
32	DTT	natural abundance	1 mM
33	D2O	natural abundance	100 %

12 3D CBCA(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
21	Tfb1	natural abundance	1.25 mM
22	Rad2	[U-100% 13C; U-100% 15N]	1 mM
23	sodium phosphate	natural abundance	20 mM
24	EDTA	natural abundance	1 mM
25	DTT	natural abundance	1 mM
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

13 3D HNCO

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
21	Tfb1	natural abundance	1.25 mM
22	Rad2	[U-100% 13C; U-100% 15N]	1 mM
23	sodium phosphate	natural abundance	20 mM
24	EDTA	natural abundance	1 mM
25	DTT	natural abundance	1 mM
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

14 3D HNCACB

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
21	Tfb1	natural abundance	1.25 mM
22	Rad2	[U-100% 13C; U-100% 15N]	1 mM
23	sodium phosphate	natural abundance	20 mM
24	EDTA	natural abundance	1 mM
25	DTT	natural abundance	1 mM
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

15 3D H(CCO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
21	Tfb1	natural abundance	1.25 mM
22	Rad2	[U-100% 13C; U-100% 15N]	1 mM
23	sodium phosphate	natural abundance	20 mM
24	EDTA	natural abundance	1 mM
25	DTT	natural abundance	1 mM
26	H2O	natural abundance	90 %
27	D2O	natural abundance	10 %

16 3D HCCH-COSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
28	Tfb1	natural abundance	1.25 mM
29	Rad2	[U-100% 13C; U-100% 15N]	1 mM
30	sodium phosphate	natural abundance	20 mM
31	EDTA	natural abundance	1 mM
32	DTT	natural abundance	1 mM
33	D2O	natural abundance	100 %

17 3D 1H-15N NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
34	Tfb1	natural abundance	1.25 mM
35	Rad2	[U-100% 15N]	1 mM
36	sodium phosphate	natural abundance	20 mM
37	EDTA	natural abundance	1 mM
38	DTT	natural abundance	1 mM
39	H2O	natural abundance	90 %
40	D2O	natural abundance	10 %

18 3D 1H-13C NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5

#	Name	Isotope labeling	Concentration
28	Tfb1	natural abundance	1.25 mM
29	Rad2	[U-100% 13C; U-100% 15N]	1 mM
30	sodium phosphate	natural abundance	20 mM
31	EDTA	natural abundance	1 mM
32	DTT	natural abundance	1 mM
33	D2O	natural abundance	100 %

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_18229_2lox.nef
Input source #2: Coordindates	2lox.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
PSHSGAAIFEKVSGIIAINEDVSPAELTWRSTDGDKVHTVVLSTIDKLQATPASSEKMMLRLIGKVDESKKRKDNEGNEVVPKPQRHMFSFNNRTVMDNI
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
PSHSGAAIFEKVSGIIAINEDVSPAELTWRSTDGDKVHTVVLSTIDKLQATPASSEKMMLRLIGKVDESKKRKDNEGNEVVPKPQRHMFSFNNRTVMDNI

-------110---------
KMTLQQIISRYKDADGNSS
|||||||||||||||||||
KMTLQQIISRYKDADGNSS

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

640-----650-------660-------670-------680-------690-
GSEILERESEKESSNDENKDDDLEVLSEELFEDVPTKSQISKEAEDNDSRKY
||||||||||||||||||||||||||||||||||||||||||||||||||||
GSEILERESEKESSNDENKDDDLEVLSEELFEDVPTKSQISKEAEDNDSRKY
--------10--------20--------30--------40--------50--

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	119	0	0	100.0
B	B	52	0	0	100.0

Content subtype: combined_18229_2lox.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1789		100.0 (chain: A, length: 119), 86.5 (chain: B, length: 52)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	OK	2259 (1)	noe	96.6 (chain: A, length: 119), 38.5 (chain: B, length: 52)
2	Distance restraints	CNS/XPLOR_distance_constraints_4	Warning	36 (1)	hbond	18.5 (chain: A, length: 119)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_3	Warning	158 (158)	.	73.1 (chain: A, length: 119), 23.1 (chain: B, length: 52)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 119, Sequence coverage: 100.0%
  - Entity_assembly_ID: B, Chain length: 52, Sequence coverage: 86.5%
- Total number of assignments
  - ¹H chemical shifts: 926
  - ¹³C chemical shifts: 690
  - ¹⁵N chemical shifts: 173
- Completeness of assignments
  - Entity_assemble_ID: A
  - Entity_assemble_ID: B
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
  - Chain_ID: B

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	718	709	98.7
¹³C chemical shifts	527	510	96.8
¹⁵N chemical shifts	132	129	97.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	239	239	100.0
¹³C chemical shifts	238	233	97.9
¹⁵N chemical shifts	114	114	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	479	470	98.1
¹³C chemical shifts	289	277	95.8
¹⁵N chemical shifts	18	15	83.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	67	67	100.0
¹³C chemical shifts	67	66	98.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	31	27	87.1
¹³C chemical shifts	30	23	76.7
¹⁵N chemical shifts	1	1	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	312	217	69.6
¹³C chemical shifts	221	180	81.4
¹⁵N chemical shifts	57	44	77.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	104	74	71.2
¹³C chemical shifts	104	85	81.7
¹⁵N chemical shifts	51	39	76.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	208	143	68.8
¹³C chemical shifts	117	95	81.2
¹⁵N chemical shifts	6	5	83.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	18	16	88.9
¹³C chemical shifts	18	14	77.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	9	4	44.4
¹³C chemical shifts	9	4	44.4

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 119, Sequence coverage: 96.6%
  - Entity_assembly_ID: B, Chain length: 52, Sequence coverage: 38.5%
- Total number of restraints: 2259
- Weight of restraints: 1.0 (2259)
- Potential type of restraints: square-well-parabolic (2259)
- Range of distance target values: 2.3, 4.5 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_4
  - Status: Warning
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 119, Sequence coverage: 18.5%
  - Total number of restraints: 36
  - Weight of restraints: 1.0 (36)
  - Potential type of restraints: square-well-parabolic (36)
  - Range of distance target values: 1.9, 2.9 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
      None
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_3
- Status: Warning
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 119, Sequence coverage: 73.1%
  - Entity_assembly_ID: B, Chain length: 52, Sequence coverage: 23.1%
- Total number of restraints: 158
- Total number of restraints per polymer type: 158
- Weight of restraints: 1.0 (158)
- Potential type of restraints: square-well-parabolic (158)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords Rad2, Tfb1, TFIIH

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Found 1 Document (1.41 seconds)

BMRB: 18229

Sample #1

Sample #2

Sample #3

Sample #4

Sample #5

Sample #6

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Tfb1, : 2 : 4 entities Detail

Related entities 2. Rad2, : 1 : 2 entities Detail

Interaction partners 1. Tfb1, : 49 interactors Detail

Interaction partners 2. Rad2, : 8 interactors Detail

Experiments performed 18 experiments Detail

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NMR combined restraints 5 contents Detail