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BMRB: 18242

2M5V

Backbone and sidechain 1H, 15N and 13C assignments of NLRP10 (PYNOD) pyrin domain

Authors

Chang, C., Chang, C., Su, M.

Assembly

PYNOD PYD

Entity

1. PYNOD PYD (polymer, Thiol state: unknown), 102 monomers, 11565.48 Da Detail

GAMAMAKARK PREALLWALS DLEENDFKKL KFYLRDMTLS EGQPPLARGE LEGLIPVDLA ELLISKYGEK EAVKVVLKGL KVMNLLELVD QLSHICLHDY RE

Formula weight

11565.48 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 89.7 %, Completeness (bb): 95.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.7 % (1101 of 1228)	89.9 % (578 of 643)	89.0 % (429 of 482)	91.3 % (94 of 103)
Backbone	95.9 % (579 of 604)	95.1 % (196 of 206)	96.3 % (289 of 300)	95.9 % (94 of 98)
Sidechain	85.6 % (616 of 720)	87.4 % (382 of 437)	84.2 % (234 of 278)	0.0 % (0 of 5)
Aromatic	12.5 % (8 of 64)	12.5 % (4 of 32)	12.9 % (4 of 31)	0.0 % (0 of 1)
Methyl	89.7 % (122 of 136)	92.6 % (63 of 68)	86.8 % (59 of 68)

1. PYNOD PYD

GAMAMAKARK PREALLWALS DLEENDFKKL KFYLRDMTLS EGQPPLARGE LEGLIPVDLA ELLISKYGEK EAVKVVLKGL KVMNLLELVD QLSHICLHDY RE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	PYNOD PYD	[U-13C; U-15N]		0.2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.35 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.35 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.35 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M5V, Strand ID: A Detail

Release date

2012-06-04

Citation

¹H, ¹³C and ¹⁵N resonance assignments of the pyrin domain from human PYNOD
Su, M., Chang, C., Chang, C.
Biomol. NMR Assign. (2013), 7, 141-143, PubMed 22618865 , DOI 10.1007/s12104-012-9396-8 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q86W26 released on 2004-04-13
Title NAL10_HUMAN Entity NACHT, LRR and PYD domains-containing protein 10

Related entities 1. PYNOD PYD, : 1 : 2 : 34 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC