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BMRB: 18269

2LPN

Solution Structure of N-Terminal domain of human Conserved Dopamine Neurotrophic Factor (CDNF)

Authors

Cristiane, L., Katia, C.M.S., Debora, F., Pires, J.M., Almeida, M.S.

Assembly

N-terminal domain of human CDNF

Entity

1. N-terminal domain of human CDNF (polymer, Thiol state: all disulfide bound), 105 monomers, 11945.79 Da Detail

QEAGGRPGAD CEVCKEFLNR FYKSLIDRGV NFSLDTIEKE LISFCLDTKG KENRLCYYLG ATKDAATKIL SEVTRPMSVH MPAMKICEKL KKLDSQICEL KYEKT

Formula weight

11945.79 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS45:SG	1:CYS56:CYS
2	disulfide	sing	1:CYS14:SG	1:CYS87:CYS
3	disulfide	sing	1:CYS11:SG	1:CYS98:CYS

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.9 %, Completeness (bb): 82.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.9 % (1059 of 1248)	88.7 % (582 of 656)	78.6 % (381 of 485)	89.7 % (96 of 107)
Backbone	82.7 % (516 of 624)	94.4 % (201 of 213)	72.2 % (223 of 309)	90.2 % (92 of 102)
Sidechain	88.8 % (642 of 723)	86.0 % (381 of 443)	93.5 % (257 of 275)	80.0 % (4 of 5)
Aromatic	73.7 % (56 of 76)	92.1 % (35 of 38)	55.3 % (21 of 38)
Methyl	100.0 % (108 of 108)	100.0 % (54 of 54)	100.0 % (54 of 54)

1. entity

QEAGGRPGAD CEVCKEFLNR FYKSLIDRGV NFSLDTIEKE LISFCLDTKG KENRLCYYLG ATKDAATKIL SEVTRPMSVH MPAMKICEKL KKLDSQICEL KYEKT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	CDNF	[U-100% 13C; U-100% 15N]	0.7 mM
2	D2O	[U-2H]	10 %
3	sodium azide	natural abundance	5 mM
4	MES	natural abundance	20 mM
5	sodium chloride	natural abundance	150 mM
6	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LPN, Strand ID: A Detail

Release date

2012-06-12

Citation 1

(1)H-, (13)C- and (15)N-NMR assignment of the N-terminal domain of human cerebral dopamine neurotrophic factor (CDNF)
Latge, C., Cabral, K.M.S., Almeida, M.S., Foguel, D.
Biomol. NMR Assign. (2013), 7, 101-103, PubMed 22528768 , DOI 10.1007/s12104-012-9388-8 , Abstract

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Citation 2

1H-, 13C- and 15N-NMR assignment of the N-terminal domain of human cerebral dopamine neurotrophic factor (CDNF)
Latge, C., Cabral, K.M.S., Almeida, M.S., Foguel, D.
Biomol. NMR Assignments

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Entries sharing articles BMRB: 1 entries Detail

BMRB: 19164 released on 2015-06-18
Title solution structure of cerebral dopamine neurotrophic factor (CDNF)

Related entities 1. N-terminal domain of human CDNF, : 1 : 3 : 1 : 31 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC