BMRB: 18318

Solution structure of CHCH5

Authors

Peruzzini, R., Ciofi-Baffoni, S., Banci, L., Bertini, I.

Assembly

CHCH5

Entity

1. CHCH5 (polymer, Thiol state: all disulfide bound), 113 monomers, 12676.24 Da Detail

GSHMQAALEV TARYCGRELE QYGQCVAAKP ESWQRDCHYL KMSIAQCTSS HPIIRQIRQA CAQPFEAFEE CLRQNEAAVG NCAEHMRRFL QCAEQVQPPR SPATVEAQPL PAS

Formula weight

12676.24 Da

Entity Connection

disulfide 4 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS15:SG	1:CYS47:SG
2	disulfide	sing	1:CYS25:SG	1:CYS37:SG
3	disulfide	sing	1:CYS61:SG	1:CYS92:SG
4	disulfide	sing	1:CYS71:SG	1:CYS82:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 71.7 %, Completeness: 49.0 %, Completeness (bb): 68.1 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	49.0 % (630 of 1285)	41.9 % (283 of 676)	55.5 % (271 of 488)	62.8 % (76 of 121)
Backbone	68.1 % (451 of 662)	65.8 % (146 of 222)	68.4 % (229 of 335)	72.4 % (76 of 105)
Sidechain	34.6 % (253 of 732)	30.2 % (137 of 454)	44.3 % (116 of 262)	0.0 % (0 of 16)
Aromatic	0.0 % (0 of 82)	0.0 % (0 of 41)	0.0 % (0 of 40)	0.0 % (0 of 1)
Methyl	50.0 % (49 of 98)	53.1 % (26 of 49)	46.9 % (23 of 49)

1. entity

GSHMQAALEV TARYCGRELE QYGQCVAAKP ESWQRDCHYL KMSIAQCTSS HPIIRQIRQA CAQPFEAFEE CLRQNEAAVG NCAEHMRRFL QCAEQVQPPR SPATVEAQPL PAS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 15N]		0.5-1 mM
2	potassium phosphate	natural abundance		50 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
5	entity	[U-100% 13C; U-100% 15N]		0.5-1 mM
6	potassium phosphate	natural abundance		50 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2LQL, Strand ID: A Detail

---

Release date

2012-09-13

Citation

Structural characterization of CHCHD5 and CHCHD7: two atypical human twin CX9C proteins
Banci, L., Bertini, I., Ciofi-Baffoni, S., Jaiswal, D., Neri, S., Peruzzini, R., Winkelmann, J.
J. Struct. Biol. (2012), 180, 190-200, PubMed 22842048 , DOI 10.1016/j.jsb.2012.07.007 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 2 entries Detail

Related entities 1. CHCH5, : 1 : 2 : 6 entities Detail

Interaction partners 1. CHCH5, : 4 interactors Detail

Experiments performed 13 experiments Detail