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BMRB: 18328

2LQT

Solution structure of CHCHD7

Authors

Winkelmann, J., Ciofi-Baffoni, S., Banci, L., Bertini, I.

Assembly

CHCHD7

Entity

1. CHCHD7 (polymer, Thiol state: all disulfide bound), 85 monomers, 10095.38 Da Detail

MPSVTQRLRD PDINPCLSES DASTRCLDEN NYDRERCSTY FLRYKNCRRF WNSIVMQRRK NGVKPFMPTA AERDEILRAV GNMPY

Formula weight

10095.38 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS16:SG	1:CYS47:SG
2	disulfide	sing	1:CYS26:SG	1:CYS37:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.9 %, Completeness: 75.5 %, Completeness (bb): 83.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.5 % (765 of 1013)	75.0 % (404 of 539)	74.3 % (286 of 385)	84.3 % (75 of 89)
Backbone	83.3 % (415 of 498)	84.9 % (141 of 166)	82.2 % (208 of 253)	83.5 % (66 of 79)
Sidechain	70.2 % (420 of 598)	70.5 % (263 of 373)	68.8 % (148 of 215)	90.0 % (9 of 10)
Aromatic	18.9 % (14 of 74)	29.7 % (11 of 37)	5.6 % (2 of 36)	100.0 % (1 of 1)
Methyl	84.4 % (54 of 64)	84.4 % (27 of 32)	84.4 % (27 of 32)

1. entity

MPSVTQRLRD PDINPCLSES DASTRCLDEN NYDRERCSTY FLRYKNCRRF WNSIVMQRRK NGVKPFMPTA AERDEILRAV GNMPY

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	CHCHD7	[U-100% 15N]	0.5 ~ 1.0 mM
2	potassium phosphate	natural abundance	50 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
5	CHCHD7	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
6	potassium phosphate	natural abundance	50 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methylene carbons	69.3 ppm	external	direct	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methylene carbons	69.3 ppm	external	direct	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methylene carbons	69.3 ppm	external	direct	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.29 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	dioxane	methylene carbons	69.3 ppm	external	direct	1.0
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.82 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LQT, Strand ID: A Detail

Release date

2012-09-13

Citation

Structural characterization of CHCHD5 and CHCHD7: two atypical human twin CX9C proteins
Banci, L., Bertini, I., Ciofi-Baffoni, S., Jaiswal, D., Neri, S., Peruzzini, R., Winkelmann, J.
J. Struct. Biol. (2012), 180, 190-200, PubMed 22842048 , DOI 10.1016/j.jsb.2012.07.007 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 2 entries Detail

  BMRB: 18318 released on 2012-09-13
    Title Solution structure of CHCH5
  Swiss-Prot: Q9BSY4 released on 2005-04-12
    Title CHCH5_HUMAN Entity Coiled-coil-helix-coiled-coil-helix domain-containing protein 5
  Swiss-Prot: Q9BUK0 released on 2005-04-12
    Title CHCH7_HUMAN Entity Coiled-coil-helix-coiled-coil-helix domain-containing protein 7

Related entities 1. CHCHD7, : 1 : 2 : 8 entities Detail

Interaction partners 1. CHCHD7, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC