BMRB: 18336

Structure of the RNA claw of the DNA packaging motor of bacteriophage 29

Authors

Harjes, E.J., Matsuo, H.J., Kitamura, A.J.

Assembly

RNA (27-MER)

Entity

1. RNA (27-MER) (polymer, Thiol state: not present), 27 monomers, 8649.098 Da Detail

GGACUUCCAU UGCUUCGGCA AAAGUCC

Formula weight

8649.098 Da

Source organism

Staphylococcus virus 29

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 63.4 %, Completeness (bb): 53.2 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	63.4 % (276 of 435)	85.8 % (205 of 239)	37.7 % (69 of 183)	15.4 % (2 of 13)
Suger, PO4	53.2 % (158 of 297)	82.7 % (134 of 162)	17.8 % (24 of 135)
Nucleobase	85.5 % (118 of 138)	92.2 % (71 of 77)	93.8 % (45 of 48)	15.4 % (2 of 13)
Aromatic	86.9 % (106 of 122)	96.7 % (59 of 61)	93.8 % (45 of 48)	15.4 % (2 of 13)

1. RNA (27-MER)

GGACUUCCAU UGCUUCGGCA AAAGUCC

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	RNA (27-MER)	[U-100% 13C; U-100% 15N]		300 uM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

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Release date

2012-07-29

Citation

Structure of the RNA claw of the DNA packaging motor of bacteriophage Φ29
Harjes, E., Kitamura, A., Zhao, W., Morais, M.C., Jardine, P.J., Grimes, S., Matsuo, H.
Nucleic Acids Res. (2012), 40, 9953-9963, PubMed 22879380 , DOI 10.1093/nar/gks724 , Abstract

Related entities 1. RNA (27-MER), : 1 entities Detail

Experiments performed 5 experiments Detail