BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	81.0 % (934 of 1153)	78.9 % (472 of 598)	83.3 % (380 of 456)	82.8 % (82 of 99)
Backbone	87.9 % (503 of 572)	85.8 % (163 of 190)	89.0 % (258 of 290)	89.1 % (82 of 92)
Sidechain	76.4 % (517 of 677)	75.7 % (309 of 408)	79.4 % (208 of 262)	0.0 % (0 of 7)
Aromatic	2.7 % (2 of 74)	2.7 % (1 of 37)	2.7 % (1 of 37)
Methyl	95.9 % (117 of 122)	93.4 % (57 of 61)	98.4 % (60 of 61)

1. Get5 ubl

AHHHHHHVDD DDKMDNAAVH LTLKKIQAPK FSIEHDFSPS DTILQIKQHL ISEEKASHIS EIKLLLKGKV LHDNLFLSDL KVTPANSTIT VMIKPNP

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6, Details S. cerevisiae Get5 UBL domain (70-152 of full-length protein) with 14 amino acid tag MAHHHHHHVDDDDKdnaavhltlkkiqapkfsiehdfspsdtilqikqhliseekashiseiklllkgkvlhdnlflsdlkvtpanstitvmikpnp

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6, Details S. cerevisiae Get5 UBL domain (70-152 of full-length protein) with 14 amino acid tag MAHHHHHHVDDDDKdnaavhltlkkiqapkfsiehdfspsdtilqikqhliseekashiseiklllkgkvlhdnlflsdlkvtpanstitvmikpnp

#	Name	Isotope labeling	Concentration
7	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
8	MES	natural abundance	100 mM
9	potassium chloride	natural abundance	150 mM
10	TCEP	natural abundance	0.5 mM
11	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.47 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 9 models in PDB: 4ASW, Strand ID: C Detail

Release date

2012-11-14

Citation

1H, 13C and 15N assignments of Sgt2 N-terminal dimerisation domain and its binding partner, Get5 Ubiquitin-like domain
Simon, A.C., Simpson, P.J., Hawthorne, W., Hale, L.R., Goldstone, R.M., Isaacson, R.L.
Biomol. NMR Assign. (2013), 7, 271-274, PubMed 23001946 , DOI 10.1007/s12104-012-9425-7 , Abstract

Related entities 1. Get5 UBL domain, : 1 : 1 : 5 : 6 entities Detail

Interaction partners 1. Get5 UBL domain, : 15 interactors Detail

More details for 15 interactors identified by 5 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6, Details S. cerevisiae Get5 UBL domain (70-152 of full-length protein) with 14 amino acid tag MAHHHHHHVDDDDKdnaavhltlkkiqapkfsiehdfspsdtilqikqhliseekashiseiklllkgkvlhdnlflsdlkvtpanstitvmikpnp

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

3 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

4 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

5 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

6 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

7 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

8 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6, Details S. cerevisiae Get5 UBL domain (70-152 of full-length protein) with 14 amino acid tag MAHHHHHHVDDDDKdnaavhltlkkiqapkfsiehdfspsdtilqikqhliseekashiseiklllkgkvlhdnlflsdlkvtpanstitvmikpnp

#	Name	Isotope labeling	Concentration
7	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
8	MES	natural abundance	100 mM
9	potassium chloride	natural abundance	150 mM
10	TCEP	natural abundance	0.5 mM
11	D2O	natural abundance	100 %

9 3D (H)CCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
7	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
8	MES	natural abundance	100 mM
9	potassium chloride	natural abundance	150 mM
10	TCEP	natural abundance	0.5 mM
11	D2O	natural abundance	100 %

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_18342_4asw.nef
Input source #2: Coordindates	4asw.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Error

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90--
MAHHHHHHVDDDDMMSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSASRVPES
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MAHHHHHHVDDDDMMSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSASRVPES

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

--------10--------20--------30--------40--------50--------60--------70--------80--------90--
MAHHHHHHVDDDDMMSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSASRVPES
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MAHHHHHHVDDDDMMSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSASRVPES

Entity_assembly_ID (NMR): C vs Auth_asym_ID (model): C

---20--------30--------40--------50--------60--------70--------80--------90--------
DNAAVHLTLKKIQAPKFSIEHDFSPSDTILQIKQHLISEEKASHISEIKLLLKGKVLHDNLFLSDLKVTPANSTITVMIKPNP
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
DNAAVHLTLKKIQAPKFSIEHDFSPSDTILQIKQHLISEEKASHISEIKLLLKGKVLHDNLFLSDLKVTPANSTITVMIKPNP
--------10--------20--------30--------40--------50--------60--------70--------80---

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Sequence coverage (%)
A	A	92	100.0
B	B	92	100.0
C	C	83	100.0

Content subtype: combined_18342_4asw.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assignments_get5_ubl_minus1	Error	945		7.6 (chain: A, length: 92), 96.4 (chain: C, length: 83)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	20 (1)	undefined	4.8 (chain: C, length: 83)

Assigned chemical shifts

Saveframe: assignments_get5_ubl_minus1
- Status: Error
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 7.6%
  - Entity_assembly_ID: C, Chain length: 83, Sequence coverage: 96.4%
- Total number of assignments
  - ¹H chemical shifts: 479
  - ¹³C chemical shifts: 384
  - ¹⁵N chemical shifts: 82
- Completeness of assignments
  - Entity_assemble_ID: C
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
  - Chain_ID: C

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	521	442	84.8
¹³C chemical shifts	395	352	89.1
¹⁵N chemical shifts	85	76	89.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	162	155	95.7
¹³C chemical shifts	166	159	95.8
¹⁵N chemical shifts	78	76	97.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	359	287	79.9
¹³C chemical shifts	229	193	84.3
¹⁵N chemical shifts	7	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	59	58	98.3
¹³C chemical shifts	59	58	98.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	25	0	0.0
¹³C chemical shifts	25	0	0.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: undefined
- Sequence coverage of experimental data
  - Entity_assembly_ID: C, Chain length: 83, Sequence coverage: 4.8%
- Total number of restraints: 19
- Weight of restraints: 1.0 (19)
- Potential type of restraints: square-well-parabolic (19)
- Range of distance target values: 3.6, 3.6 (Å)
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
  - Chain_ID: C
- Distance restraints on contact map

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.235 seconds)

BMRB: 18342

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Get5 UBL domain, : 1 : 1 : 5 : 6 entities Detail

Interaction partners 1. Get5 UBL domain, : 15 interactors Detail

Experiments performed 9 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

NMR combined restraints 3 contents Detail