BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	81.0 % (934 of 1153)	78.9 % (472 of 598)	83.3 % (380 of 456)	82.8 % (82 of 99)
Backbone	87.9 % (503 of 572)	85.8 % (163 of 190)	89.0 % (258 of 290)	89.1 % (82 of 92)
Sidechain	76.4 % (517 of 677)	75.7 % (309 of 408)	79.4 % (208 of 262)	0.0 % (0 of 7)
Aromatic	2.7 % (2 of 74)	2.7 % (1 of 37)	2.7 % (1 of 37)
Methyl	95.9 % (117 of 122)	93.4 % (57 of 61)	98.4 % (60 of 61)

1. Get5 ubl

AHHHHHHVDD DDKMDNAAVH LTLKKIQAPK FSIEHDFSPS DTILQIKQHL ISEEKASHIS EIKLLLKGKV LHDNLFLSDL KVTPANSTIT VMIKPNP

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6, Details S. cerevisiae Get5 UBL domain (70-152 of full-length protein) with 14 amino acid tag MAHHHHHHVDDDDKdnaavhltlkkiqapkfsiehdfspsdtilqikqhliseekashiseiklllkgkvlhdnlflsdlkvtpanstitvmikpnp

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6, Details S. cerevisiae Get5 UBL domain (70-152 of full-length protein) with 14 amino acid tag MAHHHHHHVDDDDKdnaavhltlkkiqapkfsiehdfspsdtilqikqhliseekashiseiklllkgkvlhdnlflsdlkvtpanstitvmikpnp

#	Name	Isotope labeling	Concentration
7	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
8	MES	natural abundance	100 mM
9	potassium chloride	natural abundance	150 mM
10	TCEP	natural abundance	0.5 mM
11	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.47 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 9 models in PDB: 4ASW, Strand ID: C Detail

Release date

2012-11-14

Citation

1H, 13C and 15N assignments of Sgt2 N-terminal dimerisation domain and its binding partner, Get5 Ubiquitin-like domain
Simon, A.C., Simpson, P.J., Hawthorne, W., Hale, L.R., Goldstone, R.M., Isaacson, R.L.
Biomol. NMR Assign. (2013), 7, 271-274, PubMed 23001946 , DOI 10.1007/s12104-012-9425-7 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 18341 released on 2012-11-14
Title Sgt2_NT

Related entities 1. Get5 UBL domain, : 1 : 1 : 5 : 6 entities Detail

Interaction partners 1. Get5 UBL domain, : 15 interactors Detail

More details for 15 interactors identified by 5 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6, Details S. cerevisiae Get5 UBL domain (70-152 of full-length protein) with 14 amino acid tag MAHHHHHHVDDDDKdnaavhltlkkiqapkfsiehdfspsdtilqikqhliseekashiseiklllkgkvlhdnlflsdlkvtpanstitvmikpnp

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

3 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

4 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

5 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

6 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

7 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
2	MES	natural abundance	100 mM
3	potassium chloride	natural abundance	150 mM
4	TCEP	natural abundance	0.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

8 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6, Details S. cerevisiae Get5 UBL domain (70-152 of full-length protein) with 14 amino acid tag MAHHHHHHVDDDDKdnaavhltlkkiqapkfsiehdfspsdtilqikqhliseekashiseiklllkgkvlhdnlflsdlkvtpanstitvmikpnp

#	Name	Isotope labeling	Concentration
7	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
8	MES	natural abundance	100 mM
9	potassium chloride	natural abundance	150 mM
10	TCEP	natural abundance	0.5 mM
11	D2O	natural abundance	100 %

9 3D (H)CCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
7	Get5_ubl	[U-98% 13C; U-98% 15N]	700 uM
8	MES	natural abundance	100 mM
9	potassium chloride	natural abundance	150 mM
10	TCEP	natural abundance	0.5 mM
11	D2O	natural abundance	100 %

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_18342_4asw.nef
Input source #2: Coordindates	4asw.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Error

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90--
MAHHHHHHVDDDDMMSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSASRVPES
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MAHHHHHHVDDDDMMSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSASRVPES

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

--------10--------20--------30--------40--------50--------60--------70--------80--------90--
MAHHHHHHVDDDDMMSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSASRVPES
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MAHHHHHHVDDDDMMSASKEEIAALIVNYFSSIVEKKEISEDGADSLNVAMDCISEAFGFEREAVSGILGKSEFKGQHLADILNSASRVPES

Entity_assembly_ID (NMR): C vs Auth_asym_ID (model): C

---20--------30--------40--------50--------60--------70--------80--------90--------
DNAAVHLTLKKIQAPKFSIEHDFSPSDTILQIKQHLISEEKASHISEIKLLLKGKVLHDNLFLSDLKVTPANSTITVMIKPNP
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
DNAAVHLTLKKIQAPKFSIEHDFSPSDTILQIKQHLISEEKASHISEIKLLLKGKVLHDNLFLSDLKVTPANSTITVMIKPNP
--------10--------20--------30--------40--------50--------60--------70--------80---

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Sequence coverage (%)
A	A	92	100.0
B	B	92	100.0
C	C	83	100.0

Content subtype: combined_18342_4asw.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assignments_get5_ubl_minus1	Error	945		7.6 (chain: A, length: 92), 96.4 (chain: C, length: 83)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	20 (1)	undefined	4.8 (chain: C, length: 83)

Assigned chemical shifts

Saveframe: assignments_get5_ubl_minus1
- Status: Error
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 7.6%
  - Entity_assembly_ID: C, Chain length: 83, Sequence coverage: 96.4%
- Total number of assignments
  - ¹H chemical shifts: 479
  - ¹³C chemical shifts: 384
  - ¹⁵N chemical shifts: 82
- Completeness of assignments
  - Entity_assemble_ID: C
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
  - Chain_ID: C

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	521	442	84.8
¹³C chemical shifts	395	352	89.1
¹⁵N chemical shifts	85	76	89.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	162	155	95.7
¹³C chemical shifts	166	159	95.8
¹⁵N chemical shifts	78	76	97.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	359	287	79.9
¹³C chemical shifts	229	193	84.3
¹⁵N chemical shifts	7	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	59	58	98.3
¹³C chemical shifts	59	58	98.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	25	0	0.0
¹³C chemical shifts	25	0	0.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: undefined
- Sequence coverage of experimental data
  - Entity_assembly_ID: C, Chain length: 83, Sequence coverage: 4.8%
- Total number of restraints: 19
- Weight of restraints: 1.0 (19)
- Potential type of restraints: square-well-parabolic (19)
- Range of distance target values: 3.6, 3.6 (Å)
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
  - Chain_ID: C
- Distance restraints on contact map

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Found 1 Document (1.078 seconds)

BMRB: 18342

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Get5 UBL domain, : 1 : 1 : 5 : 6 entities Detail

Interaction partners 1. Get5 UBL domain, : 15 interactors Detail

Experiments performed 9 experiments Detail

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NMR combined restraints 3 contents Detail