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BMRB: 18374

2LRI

NMR structure of the second PHD finger of AIRE (AIRE-PHD2)

Authors

Gaetani, M., Chignola, F., Mollica, L., Quilici, G., Mannella, V., Spiliotopoulos, D., Musco, G.

Assembly

AIRE-PHD2

Entity

1. AIRE-PHD2 (polymer), 66 monomers, 6801.609 Da Detail

GAMEGQQNLA PGARCGVCGD GTDVLRCTHC AAAFHWRCHF PAGTSRPGTG LRCRSCSGDV TPAPVE

2. ZINC ION (non-polymer, Thiol state: not present), 65.409 × 2 Da

Total weight

6932.427 Da

Max. entity weight

6801.609 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.0 %, Completeness (bb): 83.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.0 % (587 of 667)	95.3 % (327 of 343)	76.1 % (197 of 259)	96.9 % (63 of 65)
Backbone	83.2 % (321 of 386)	97.1 % (133 of 137)	67.6 % (127 of 188)	100.0 % (61 of 61)
Sidechain	95.5 % (322 of 337)	94.2 % (194 of 206)	99.2 % (126 of 127)	50.0 % (2 of 4)
Aromatic	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (21 of 21)	100.0 % (1 of 1)
Methyl	100.0 % (54 of 54)	100.0 % (27 of 27)	100.0 % (27 of 27)

1. AIRE-PHD2

GAMEGQQNLA PGARCGVCGD GTDVLRCTHC AAAFHWRCHF PAGTSRPGTG LRCRSCSGDV TPAPVE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
1	AIRE-PHD2	[U-100% 13C; U-100% 15N]	0.4 ~ 0.8 mM
2	ZINC ION	natural abundance	50 uM
3	DTT	natural abundance	5 mM
4	sodium phosphate	natural abundance	50 mM
5	sodium chloride	natural abundance	0.15 M
6	sodium azide	natural abundance	0.02 %
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
9	AIRE-PHD2	[U-100% 13C; U-100% 15N]	0.4 ~ 0.8 mM
10	DTT	natural abundance	5 mM
11	ZINC ION	natural abundance	50 uM
12	sodium phosphate	natural abundance	50 mM
13	sodium chloride	natural abundance	0.15 M
14	sodium azide	natural abundance	0.02 %
15	D2O	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
16	AIRE-PHD2	[U-100% 15N]	0.4 ~ 0.8 mM
17	DTT	natural abundance	5 mM
18	ZINC ION	natural abundance	50 uM
19	sodium phosphate	natural abundance	50 mM
20	sodium chloride	natural abundance	0.15 M
21	sodium azide	natural abundance	0.02 %
22	D2O	natural abundance	10 %
23	H2O	natural abundance	90 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
24	AIRE-PHD2	natural abundance	0.4 ~ 0.8 mM
25	DTT	natural abundance	5 mM
26	ZINC ION	natural abundance	50 uM
27	sodium phosphate	natural abundance	50 mM
28	sodium chloride	natural abundance	0.15 M
29	sodium azide	natural abundance	0.02 %
30	D2O	natural abundance	10 %
31	H2O	natural abundance	90 %

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
32	AIRE-PHD2	natural abundance	0.4 ~ 0.8 mM
33	DTT	natural abundance	5 mM
34	ZINC ION	natural abundance	50 uM
35	sodium phosphate	natural abundance	50 mM
36	sodium chloride	natural abundance	0.15 M
37	sodium azide	natural abundance	0.02 %
38	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 50 models in PDB: 2LRI, Strand ID: C Detail

Release date

2012-10-11

Citation

AIRE-PHD fingers are structural hubs to maintain the integrity of chromatin-associated interactome
Gaetani, M., Matafora, V., Saare, M., Spiliotopoulos, D., Mollica, L., Quilici, G., Chignola, F., Mannella, V., Zucchelli, C., Peterson, P., Bachi, A., Musco, G.
Nucleic Acids Res. (2012), 40, 11756-11768, PubMed 23074189 , DOI 10.1093/nar/gks933 , Abstract

Related entities 1. AIRE-PHD2, : 1 : 2 : 9 entities Detail

Interaction partners 1. AIRE-PHD2, : 13 interactors Detail

More details for 13 interactors identified by 2 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
16	AIRE-PHD2	[U-100% 15N]	0.4 ~ 0.8 mM
17	DTT	natural abundance	5 mM
18	ZINC ION	natural abundance	50 uM
19	sodium phosphate	natural abundance	50 mM
20	sodium chloride	natural abundance	0.15 M
21	sodium azide	natural abundance	0.02 %
22	D2O	natural abundance	10 %
23	H2O	natural abundance	90 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
1	AIRE-PHD2	[U-100% 13C; U-100% 15N]	0.4 ~ 0.8 mM
2	ZINC ION	natural abundance	50 uM
3	DTT	natural abundance	5 mM
4	sodium phosphate	natural abundance	50 mM
5	sodium chloride	natural abundance	0.15 M
6	sodium azide	natural abundance	0.02 %
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

3 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
24	AIRE-PHD2	natural abundance	0.4 ~ 0.8 mM
25	DTT	natural abundance	5 mM
26	ZINC ION	natural abundance	50 uM
27	sodium phosphate	natural abundance	50 mM
28	sodium chloride	natural abundance	0.15 M
29	sodium azide	natural abundance	0.02 %
30	D2O	natural abundance	10 %
31	H2O	natural abundance	90 %

4 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
32	AIRE-PHD2	natural abundance	0.4 ~ 0.8 mM
33	DTT	natural abundance	5 mM
34	ZINC ION	natural abundance	50 uM
35	sodium phosphate	natural abundance	50 mM
36	sodium chloride	natural abundance	0.15 M
37	sodium azide	natural abundance	0.02 %
38	D2O	natural abundance	100 %

5 2D 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
24	AIRE-PHD2	natural abundance	0.4 ~ 0.8 mM
25	DTT	natural abundance	5 mM
26	ZINC ION	natural abundance	50 uM
27	sodium phosphate	natural abundance	50 mM
28	sodium chloride	natural abundance	0.15 M
29	sodium azide	natural abundance	0.02 %
30	D2O	natural abundance	10 %
31	H2O	natural abundance	90 %

6 2D 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
32	AIRE-PHD2	natural abundance	0.4 ~ 0.8 mM
33	DTT	natural abundance	5 mM
34	ZINC ION	natural abundance	50 uM
35	sodium phosphate	natural abundance	50 mM
36	sodium chloride	natural abundance	0.15 M
37	sodium azide	natural abundance	0.02 %
38	D2O	natural abundance	100 %

7 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
1	AIRE-PHD2	[U-100% 13C; U-100% 15N]	0.4 ~ 0.8 mM
2	ZINC ION	natural abundance	50 uM
3	DTT	natural abundance	5 mM
4	sodium phosphate	natural abundance	50 mM
5	sodium chloride	natural abundance	0.15 M
6	sodium azide	natural abundance	0.02 %
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

8 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
1	AIRE-PHD2	[U-100% 13C; U-100% 15N]	0.4 ~ 0.8 mM
2	ZINC ION	natural abundance	50 uM
3	DTT	natural abundance	5 mM
4	sodium phosphate	natural abundance	50 mM
5	sodium chloride	natural abundance	0.15 M
6	sodium azide	natural abundance	0.02 %
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

9 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
1	AIRE-PHD2	[U-100% 13C; U-100% 15N]	0.4 ~ 0.8 mM
2	ZINC ION	natural abundance	50 uM
3	DTT	natural abundance	5 mM
4	sodium phosphate	natural abundance	50 mM
5	sodium chloride	natural abundance	0.15 M
6	sodium azide	natural abundance	0.02 %
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

10 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
1	AIRE-PHD2	[U-100% 13C; U-100% 15N]	0.4 ~ 0.8 mM
2	ZINC ION	natural abundance	50 uM
3	DTT	natural abundance	5 mM
4	sodium phosphate	natural abundance	50 mM
5	sodium chloride	natural abundance	0.15 M
6	sodium azide	natural abundance	0.02 %
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

11 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
1	AIRE-PHD2	[U-100% 13C; U-100% 15N]	0.4 ~ 0.8 mM
2	ZINC ION	natural abundance	50 uM
3	DTT	natural abundance	5 mM
4	sodium phosphate	natural abundance	50 mM
5	sodium chloride	natural abundance	0.15 M
6	sodium azide	natural abundance	0.02 %
7	D2O	natural abundance	10 %
8	H2O	natural abundance	90 %

12 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
9	AIRE-PHD2	[U-100% 13C; U-100% 15N]	0.4 ~ 0.8 mM
10	DTT	natural abundance	5 mM
11	ZINC ION	natural abundance	50 uM
12	sodium phosphate	natural abundance	50 mM
13	sodium chloride	natural abundance	0.15 M
14	sodium azide	natural abundance	0.02 %
15	D2O	natural abundance	100 %

13 3D HNHA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
16	AIRE-PHD2	[U-100% 15N]	0.4 ~ 0.8 mM
17	DTT	natural abundance	5 mM
18	ZINC ION	natural abundance	50 uM
19	sodium phosphate	natural abundance	50 mM
20	sodium chloride	natural abundance	0.15 M
21	sodium azide	natural abundance	0.02 %
22	D2O	natural abundance	10 %
23	H2O	natural abundance	90 %

14 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
16	AIRE-PHD2	[U-100% 15N]	0.4 ~ 0.8 mM
17	DTT	natural abundance	5 mM
18	ZINC ION	natural abundance	50 uM
19	sodium phosphate	natural abundance	50 mM
20	sodium chloride	natural abundance	0.15 M
21	sodium azide	natural abundance	0.02 %
22	D2O	natural abundance	10 %
23	H2O	natural abundance	90 %

15 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 295 K, pH 6.5, Details 50mM sodium phosphate pH6.5, 0.15M NaCl, 5mM DTT, 50 M ZnCl2, 0.02% NaN3

#	Name	Isotope labeling	Concentration
9	AIRE-PHD2	[U-100% 13C; U-100% 15N]	0.4 ~ 0.8 mM
10	DTT	natural abundance	5 mM
11	ZINC ION	natural abundance	50 uM
12	sodium phosphate	natural abundance	50 mM
13	sodium chloride	natural abundance	0.15 M
14	sodium azide	natural abundance	0.02 %
15	D2O	natural abundance	100 %

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_18374_2lri.nef
Input source #2: Coordindates	2lri.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	True (see coodinates for details)
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:35:HIS:ND1	2:1:ZN:ZN	unknown	unknown	n/a
1:56:CYS:SG	2:2:ZN:ZN	unknown	unknown	n/a
1:27:CYS:SG	2:2:ZN:ZN	unknown	unknown	n/a
1:30:CYS:SG	2:2:ZN:ZN	unknown	unknown	n/a
1:38:CYS:SG	2:1:ZN:ZN	unknown	unknown	n/a
1:53:CYS:SG	2:2:ZN:ZN	unknown	unknown	n/a
1:18:CYS:SG	2:1:ZN:ZN	unknown	unknown	n/a
1:15:CYS:SG	2:1:ZN:ZN	unknown	unknown	n/a

Non-standard residues

Chain_ID	Seq_ID	Comp_ID	Chem_comp_name	Experimental evidences
A	1	ZN	ZINC ION	None
A	2	ZN	ZINC ION	None

Sequence alignments

Entity_assembly_ID (NMR): C vs Auth_asym_ID (model): C

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
C	C	66	0	0	100.0

Content subtype: combined_18374_2lri.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Covalent bonds	assembly	OK	8		No information
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	581		100.0 (chain: C, length: 66)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	3 (1)	noe	6.1 (chain: C, length: 66)
2	Distance restraints	CNS/XPLOR_distance_constraints_3	Warning	1111 (1)	noe	97.0 (chain: C, length: 66)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_4	OK	80 (80)	.	87.9 (chain: C, length: 66)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: C, Chain length: 66, Sequence coverage: 100.0%
- Total number of assignments
  - ¹³C chemical shifts: 192
  - ¹H chemical shifts: 327
  - ¹⁵N chemical shifts: 62
- Completeness of assignments
  - Entity_assemble_ID: C
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: C

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
35	HIS	HE2	11.564
35	HIS	NE2	105.359

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	259	192	74.1
¹H chemical shifts	343	326	95.0
¹⁵N chemical shifts	71	61	85.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	132	66	50.0
¹H chemical shifts	137	133	97.1
¹⁵N chemical shifts	61	60	98.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	127	126	99.2
¹H chemical shifts	206	193	93.7
¹⁵N chemical shifts	10	1	10.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	28	27	96.4
¹H chemical shifts	28	27	96.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	21	21	100.0
¹H chemical shifts	22	22	100.0
¹⁵N chemical shifts	1	1	100.0

Covalent bonds

Saveframe: assembly
- Status: OK

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: C, Chain length: 66, Sequence coverage: 6.1%
- Total number of restraints: 3
- Weight of restraints: 1.0 (3)
- Potential type of restraints: square-well-parabolic (3)
- Range of distance target values: 2.21, 2.21 (Å)
- Distance restraints per residue
  - Chain_ID: A
    None
  - Chain_ID: C
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_3
  - Status: Warning
  - Experiment type: noe
  - Sequence coverage of experimental data
    - Entity_assembly_ID: C, Chain length: 66, Sequence coverage: 97.0%
  - Total number of restraints: 1108
  - Weight of restraints: 1.0 (1108)
  - Potential type of restraints: square-well-parabolic (1108)
  - Range of distance target values: 1.57, 5.55 (Å)
  - Histogram of distance target values
  - Histogram of discrepancy in distance target values
  - Distance restraints per residue
    - Chain_ID: A
      None
    - Chain_ID: C
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_4
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: C, Chain length: 66, Sequence coverage: 87.9%
- Total number of restraints: 80
- Total number of restraints per polymer type: 80
- Weight of restraints: 1.0 (80)
- Potential type of restraints: square-well-parabolic (80)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: C

Keywords AIRE, APECED, PHD finger, Zn binding protein domain

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Found 1 Document (0.947 seconds)

BMRB: 18374

Sample #1

Sample #2

Sample #3

Sample #4

Sample #5

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. AIRE-PHD2, : 1 : 2 : 9 entities Detail

Interaction partners 1. AIRE-PHD2, : 13 interactors Detail

Experiments performed 15 experiments Detail

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NMR combined restraints 5 contents Detail