BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	86.5 % (1260 of 1457)	84.9 % (636 of 749)	86.0 % (484 of 563)	96.6 % (140 of 145)
Backbone	97.2 % (737 of 758)	97.4 % (258 of 265)	97.3 % (360 of 370)	96.7 % (119 of 123)
Sidechain	78.4 % (637 of 813)	78.1 % (378 of 484)	77.5 % (238 of 307)	95.5 % (21 of 22)
Aromatic	29.4 % (37 of 126)	52.4 % (33 of 63)	0.0 % (0 of 59)	100.0 % (4 of 4)
Methyl	92.3 % (131 of 142)	93.0 % (66 of 71)	91.5 % (65 of 71)

1. Target Recognition Domain of Zoocin A

MRGSHHHHHH GSEPTTPTTN LKIYKVDDLQ KINGIWQVRN NILVPTDFTW VDNGIAADDV IEVTSNGTRT SDQVLQKGGY FVINPNNVKS VGTPMKGSGG LSWAQVNFTT GGNVWLNTTS KDNLLYGK

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 297 (±2) K, pH 7.0 (±0.1), Details pH 7.0

#	Name	Isotope labeling	Concentration
1	Target Recognition Domain of Zoocin A	[U-95% 13C; U-90% 15N]	2.4 (±0.1) mM
2	potassium phosphate	natural abundance	10 (±0.1) mM
3	sodium azide	natural abundance	0.1 (±0.01) mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 297 (±2) K, pH 7.0 (±0.1), Details multiple samples of N-15 enriched protein were employed in this study at pH values ranging from 5.6 to 7.0.

#	Name	Isotope labeling	Concentration
6	Target Recognition Domain of Zoocin A	[U-90% 15N]	0.7 ~ 1.4 mM
7	potassium phosphate	natural abundance	10 mM
8	sodium azide	natural abundance	0.1 mM
9	H2O	natural abundance	95 %
10	D2O	natural abundance	5 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297 (±2) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
11	Target Recognition Domain of Zoocin A	natural abundance	0.7 ~ 1.4 mM
12	potassium phosphate	natural abundance	10 mM
13	sodium azide	natural abundance	0.1 mM
14	H2O	natural abundance	90 %
15	D2O	natural abundance	10 %

Sample #4

Solvent system 99.8% D2O, Pressure 1 atm, Temperature 297 (±2) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
16	Target Recognition Domain of Zoocin A	natural abundance	0.7 ~ 1.4 mM
17	potassium phosphate	natural abundance	10 mM
18	sodium azide	natural abundance	0.1 mM
19	D2O	natural abundance	99.8 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.5 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LS0, Strand ID: 1 Detail

Release date

2012-04-11

Citation

Solution structure of the recombinant target recognition domain of zoocin A
Chen, Y., Simmonds, R.S., Young, J.K., Timkovich, R.
Proteins (2013), 81, 722-727, PubMed 23184858 , DOI 10.1002/prot.24224 , Abstract