BMRBj - BMREntryMaster

Found 1 Document (0.902 seconds)

BMRB: 18416

2LWP

1H,15N and 13C backbone and side chain chemical shifts of the ubiquitin homology domain of mouse BAG-1

Authors

Huang, H., Yu, C.

Assembly

ubiquitin homology of mouse BAG-1

Entity

1. ubiquitin homology of mouse BAG-1 (polymer, Thiol state: all free), 97 monomers, 10789.32 Da Detail

MAKTEEMVQT EEMETPRLSV IVTHSNERYD LLVTPQQGNS EPVVQDLAQL VEEATGVPLP FQKLIFKGKS LKEMETPLSA LGMQNGCRVM LIGEKSN

Formula weight

10789.32 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 90.1 %, Completeness (bb): 94.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.1 % (1017 of 1129)	89.9 % (532 of 592)	89.4 % (389 of 435)	94.1 % (96 of 102)
Backbone	94.0 % (536 of 570)	93.3 % (181 of 194)	94.7 % (270 of 285)	93.4 % (85 of 91)
Sidechain	87.7 % (570 of 650)	88.2 % (351 of 398)	86.3 % (208 of 241)	100.0 % (11 of 11)
Aromatic	28.1 % (9 of 32)	56.3 % (9 of 16)	0.0 % (0 of 16)
Methyl	93.0 % (106 of 114)	94.7 % (54 of 57)	91.2 % (52 of 57)

1. ubiquitin homology domain of mouse BAG-1

MAKTEEMVQT EEMETPRLSV IVTHSNERYD LLVTPQQGNS EPVVQDLAQL VEEATGVPLP FQKLIFKGKS LKEMETPLSA LGMQNGCRVM LIGEKSN

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	ubiquitin homology domain of mouse BAG-1	[U-100% 13C; U-100% 15N]	1.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.35 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 18 models in PDB: 2LWP, Strand ID: A Detail

Release date

2012-09-13

Citation

Backbone and side-chain resonance assignments (¹H, ¹⁵N and ¹³C) of the ubiquitin homology domain of mouse BAG-1
Huang, H., Yu, C.
Biomol. NMR Assign. (2013), 7, 235-239, PubMed 22903788 , DOI 10.1007/s12104-012-9417-7 , Abstract

Related entities 1. ubiquitin homology of mouse BAG-1, : 1 : 3 : 14 entities Detail

Interaction partners 1. ubiquitin homology of mouse BAG-1, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC