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BMRB: 18426

2LSB

Solution-state NMR structure of the human prion protein

Authors

Biljan, I., Ilc, G., Giancin, G., Zhukov, I., Plavec, J., Legname, G.

Assembly

human prion protein

Entity

1. human prion protein (polymer, Thiol state: all disulfide bound), 142 monomers, 16147.81 Da Detail

GQGGGTHSQW NKPSKPKTNM KHMAGAAAAG AVVGGLGGYM LGSAMSRPII HFGSDYEDRY YRENMHRYPN QVYYRPMDEY SNQNNFVHDC VNITIKQHTV TTTTKGENFT ETDVKMMERV VEQMCITQYE RESQAYYQRG SS

Formula weight

16147.81 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS90:SG	1:CYS125:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 88.7 %, Completeness (bb): 84.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.7 % (1442 of 1626)	94.2 % (800 of 849)	78.9 % (490 of 621)	97.4 % (152 of 156)
Backbone	84.0 % (707 of 842)	95.6 % (280 of 293)	71.4 % (294 of 412)	97.1 % (133 of 137)
Sidechain	94.2 % (859 of 912)	93.5 % (520 of 556)	95.0 % (320 of 337)	100.0 % (19 of 19)
Aromatic	89.6 % (138 of 154)	89.6 % (69 of 77)	89.5 % (68 of 76)	100.0 % (1 of 1)
Methyl	96.1 % (98 of 102)	94.1 % (48 of 51)	98.0 % (50 of 51)

1. entity

GQGGGTHSQW NKPSKPKTNM KHMAGAAAAG AVVGGLGGYM LGSAMSRPII HFGSDYEDRY YRENMHRYPN QVYYRPMDEY SNQNNFVHDC VNITIKQHTV TTTTKGENFT ETDVKMMERV VEQMCITQYE RESQAYYQRG SS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	huPrP	[U-100% 13C; U-100% 15N]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
4	huPrP	[U-100% 13C; U-100% 15N]		1.5 mM
5	D2O	natural abundance		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LSB, Strand ID: A Detail

Release date

2012-06-17

Citation

Structural basis for the protective effect of the human prion protein carrying the dominant-negative E219K polymorphism
Biljan, I., Giachin, G., Ilc, G., Zhukov, I., Plavec, J., Legname, G.
Biochem. J. (2012), 446, 243-251, PubMed 22676969 , DOI 10.1042/BJ20111940 , Abstract

Related entities 1. human prion protein, : 1 : 5 : 14 : 11 : 75 entities Detail

Interaction partners 1. human prion protein, : 73 interactors Detail

More details for 73 interactors identified by 15 citations