BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	89.6 % (1109 of 1238)	96.9 % (619 of 639)	79.5 % (387 of 487)	92.0 % (103 of 112)
Backbone	83.5 % (516 of 618)	95.7 % (201 of 210)	72.0 % (221 of 307)	93.1 % (94 of 101)
Sidechain	96.1 % (691 of 719)	97.4 % (418 of 429)	94.6 % (264 of 279)	81.8 % (9 of 11)
Aromatic	85.2 % (75 of 88)	100.0 % (44 of 44)	69.0 % (29 of 42)	100.0 % (2 of 2)
Methyl	100.0 % (140 of 140)	100.0 % (70 of 70)	100.0 % (70 of 70)

1. entity

GSGGFRPAAP NLAGAVEFSD VKTLLKEWIT TISDPMEEDI LQVVRYCTDL IEEKDLEKLD LVIKYMKRLM QQSVESVWNM AFDFILDNVQ VVLQQTYGST LKVT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Rev1	[U-100% 15N]	0.9 ~ 1.0 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	PMSF	natural abundance	1 mM
6	EDTA	natural abundance	1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1	[U-100% 13C; U-100% 15N]	0.9 ~ 1.0 mM
10	sodium phosphate	natural abundance	25 mM
11	potassium chloride	natural abundance	100 mM
12	DTT	natural abundance	10 mM
13	PMSF	natural abundance	1 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
17	Rev1	[U-100% 13C; U-100% 15N]	1 mM
18	sodium phosphate	natural abundance	25 mM
19	potassium chloride	natural abundance	100 mM
20	DTT	natural abundance	10 mM
21	PMSF	natural abundance	1 mM
22	EDTA	natural abundance	1 mM
23	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LSG, Strand ID: A Detail

Release date

2012-06-17

Citation

Multifaceted recognition of vertebrate Rev1 by translesion polymerases ζ and κ
Wojtaszek, J., Liu, J., Wang, S., Xue, S., Walker, Y., Zhou, G.C.
J. Biol. Chem. (2012), 287, 26400-26408, PubMed 22700975 , DOI 10.1074/jbc.M112.380998 , Abstract

Related entities 1. mouse Rev1 C-terminal domain, : 1 : 4 : 21 entities Detail

Interaction partners 1. mouse Rev1 C-terminal domain, : 7 interactors Detail

More details for 7 interactors identified by 2 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Rev1	[U-100% 15N]	0.9 ~ 1.0 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	PMSF	natural abundance	1 mM
6	EDTA	natural abundance	1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1	[U-100% 13C; U-100% 15N]	0.9 ~ 1.0 mM
10	sodium phosphate	natural abundance	25 mM
11	potassium chloride	natural abundance	100 mM
12	DTT	natural abundance	10 mM
13	PMSF	natural abundance	1 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

3 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1	[U-100% 13C; U-100% 15N]	0.9 ~ 1.0 mM
10	sodium phosphate	natural abundance	25 mM
11	potassium chloride	natural abundance	100 mM
12	DTT	natural abundance	10 mM
13	PMSF	natural abundance	1 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

4 3D (HCA)CO(CA)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1	[U-100% 13C; U-100% 15N]	0.9 ~ 1.0 mM
10	sodium phosphate	natural abundance	25 mM
11	potassium chloride	natural abundance	100 mM
12	DTT	natural abundance	10 mM
13	PMSF	natural abundance	1 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

5 3D HNCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1	[U-100% 13C; U-100% 15N]	0.9 ~ 1.0 mM
10	sodium phosphate	natural abundance	25 mM
11	potassium chloride	natural abundance	100 mM
12	DTT	natural abundance	10 mM
13	PMSF	natural abundance	1 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

6 3D HN(CO)CA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1	[U-100% 13C; U-100% 15N]	0.9 ~ 1.0 mM
10	sodium phosphate	natural abundance	25 mM
11	potassium chloride	natural abundance	100 mM
12	DTT	natural abundance	10 mM
13	PMSF	natural abundance	1 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

7 3D HN(CA)CB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1	[U-100% 13C; U-100% 15N]	0.9 ~ 1.0 mM
10	sodium phosphate	natural abundance	25 mM
11	potassium chloride	natural abundance	100 mM
12	DTT	natural abundance	10 mM
13	PMSF	natural abundance	1 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

8 3D HNCO(CA)CB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1	[U-100% 13C; U-100% 15N]	0.9 ~ 1.0 mM
10	sodium phosphate	natural abundance	25 mM
11	potassium chloride	natural abundance	100 mM
12	DTT	natural abundance	10 mM
13	PMSF	natural abundance	1 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

9 3D HA(CA)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1	[U-100% 13C; U-100% 15N]	0.9 ~ 1.0 mM
10	sodium phosphate	natural abundance	25 mM
11	potassium chloride	natural abundance	100 mM
12	DTT	natural abundance	10 mM
13	PMSF	natural abundance	1 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

10 3D HA(CACO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1	[U-100% 13C; U-100% 15N]	0.9 ~ 1.0 mM
10	sodium phosphate	natural abundance	25 mM
11	potassium chloride	natural abundance	100 mM
12	DTT	natural abundance	10 mM
13	PMSF	natural abundance	1 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

11 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Rev1	[U-100% 15N]	0.9 ~ 1.0 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	PMSF	natural abundance	1 mM
6	EDTA	natural abundance	1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

12 3D 1H-13C NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
17	Rev1	[U-100% 13C; U-100% 15N]	1 mM
18	sodium phosphate	natural abundance	25 mM
19	potassium chloride	natural abundance	100 mM
20	DTT	natural abundance	10 mM
21	PMSF	natural abundance	1 mM
22	EDTA	natural abundance	1 mM
23	D2O	natural abundance	100 %

13 4D HCCONH TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1	[U-100% 13C; U-100% 15N]	0.9 ~ 1.0 mM
10	sodium phosphate	natural abundance	25 mM
11	potassium chloride	natural abundance	100 mM
12	DTT	natural abundance	10 mM
13	PMSF	natural abundance	1 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

14 4D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
17	Rev1	[U-100% 13C; U-100% 15N]	1 mM
18	sodium phosphate	natural abundance	25 mM
19	potassium chloride	natural abundance	100 mM
20	DTT	natural abundance	10 mM
21	PMSF	natural abundance	1 mM
22	EDTA	natural abundance	1 mM
23	D2O	natural abundance	100 %

15 4D 13C-HMQC-NOESY-HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
17	Rev1	[U-100% 13C; U-100% 15N]	1 mM
18	sodium phosphate	natural abundance	25 mM
19	potassium chloride	natural abundance	100 mM
20	DTT	natural abundance	10 mM
21	PMSF	natural abundance	1 mM
22	EDTA	natural abundance	1 mM
23	D2O	natural abundance	100 %

16 4D 13C-HMQC-NOESY-15N-HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Rev1	[U-100% 15N]	0.9 ~ 1.0 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	PMSF	natural abundance	1 mM
6	EDTA	natural abundance	1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_18431_2lsg.nef
Input source #2: Coordindates	2lsg.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
GSGGFRPAAPNLAGAVEFSDVKTLLKEWITTISDPMEEDILQVVRYCTDLIEEKDLEKLDLVIKYMKRLMQQSVESVWNMAFDFILDNVQVVLQQTYGST
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GSGGFRPAAPNLAGAVEFSDVKTLLKEWITTISDPMEEDILQVVRYCTDLIEEKDLEKLDLVIKYMKRLMQQSVESVWNMAFDFILDNVQVVLQQTYGST

----
LKVT
||||
LKVT

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	104	0	0	100.0

Content subtype: combined_18431_2lsg.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1081		98.1 (chain: A, length: 104)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	OK	3132 (1)	noe	97.1 (chain: A, length: 104)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_3	OK	160 (160)	.	89.4 (chain: A, length: 104)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 104, Sequence coverage: 98.1%
- Total number of assignments
  - ¹H chemical shifts: 618
  - ¹³C chemical shifts: 363
  - ¹⁵N chemical shifts: 100
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	639	618	96.7
¹³C chemical shifts	487	363	74.5
¹⁵N chemical shifts	115	100	87.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	210	200	95.2
¹³C chemical shifts	208	102	49.0
¹⁵N chemical shifts	101	91	90.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	429	418	97.4
¹³C chemical shifts	279	261	93.5
¹⁵N chemical shifts	14	9	64.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	74	73	98.6
¹³C chemical shifts	74	73	98.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	44	44	100.0
¹³C chemical shifts	42	27	64.3
¹⁵N chemical shifts	2	2	100.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 104, Sequence coverage: 97.1%
- Total number of restraints: 3132
- Weight of restraints: 1.0 (3132)
- Potential type of restraints: upper-bound-parabolic (3132)
- Range of distance target values: 2.46, 7.0 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 104, Sequence coverage: 89.4%
- Total number of restraints: 160
- Total number of restraints per polymer type: 160
- Weight of restraints: 1.0 (160)
- Potential type of restraints: square-well-parabolic (160)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords CTD, Rev1, translesion DNA polymerase, Y-family

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Found 1 Document (0.814 seconds)

BMRB: 18431

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. mouse Rev1 C-terminal domain, : 1 : 4 : 21 entities Detail

Interaction partners 1. mouse Rev1 C-terminal domain, : 7 interactors Detail

Experiments performed 16 experiments Detail

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NMR combined restraints 4 contents Detail