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BMRB: 18432

2LSI

Solution structure of polymerase-interacting domain of human Rev1 in complex with translesional synthesis polymerase kappa

Authors

Liu, D., Ryu, K., Ko, J., Choi, B.

Assembly

Rev1

Entity

1. Rev1 (polymer), 232 monomers, 27127.04 Da Detail

GHMAPNLAGA VEFNDVKTLL REWITTISDP MEEDILQVVK YCTDLIEEKD LEKLDLVIKY MKRLMQQSVE SVWNMAFDFI LDNVQVVLQQ TYGSTLKVTG SHKKSFFDKK RSERKWGHMA PNLAGAVEFN DVKTLLREWI TTISDPMEED ILQVVKYCTD LIEEKDLEKL DLVIKYMKRL MQQSVESVWN MAFDFILDNV QVVLQQTYGS TLKVTGSHKK SFFDKKRSER KW

Formula weight

27127.04 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, explicit water refinement

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 64.2 %, Completeness: 56.2 %, Completeness (bb): 60.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	56.2 % (1604 of 2852)	55.3 % (822 of 1486)	56.4 % (627 of 1112)	61.0 % (155 of 254)
Backbone	60.2 % (833 of 1384)	58.8 % (275 of 468)	60.6 % (417 of 688)	61.8 % (141 of 228)
Sidechain	53.7 % (909 of 1692)	53.7 % (547 of 1018)	53.7 % (348 of 648)	53.8 % (14 of 26)
Aromatic	37.7 % (89 of 236)	39.8 % (47 of 118)	34.8 % (39 of 112)	50.0 % (3 of 6)
Methyl	60.1 % (166 of 276)	60.9 % (84 of 138)	59.4 % (82 of 138)

1. Rev1

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Rev1	[U-99% 13C; U-99% 15N]	0.8 mM
2	Bis-Tris	natural abundance	50 mM
3	NaCl	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: -0.18 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: -0.18 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291
³¹P	DSS	methyl protons	na	indirect	0.4048086

Referencing offset: -0.2 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2LSI, Strand ID: A, B Detail

Release date

2013-04-01

Citation

Insights into the regulation of human Rev1 for translesion synthesis polymerases revealed by the structural studies on its polymerase-interacting domain
Liu, D., Ryu, K., Ko, J., Sun, D., Lim, K., Lee, J., Hwang, J., Lee, Z., Choi, B.
J. Mol. Cell Biol. (2013), 5, 204-206, PubMed 23220741 , DOI 10.1093/jmcb/mjs061 , Abstract

Related entities 1. Rev1, : 44 entities Detail

Experiments performed 10 experiments Detail

1 3D 1H-13C NOESY aromatic