BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	83.9 % (1417 of 1689)	90.4 % (794 of 878)	74.7 % (490 of 656)	85.8 % (133 of 155)
Backbone	78.0 % (668 of 856)	88.3 % (257 of 291)	68.0 % (289 of 425)	87.1 % (122 of 140)
Sidechain	90.4 % (877 of 970)	91.5 % (537 of 587)	89.4 % (329 of 368)	73.3 % (11 of 15)
Aromatic	79.3 % (92 of 116)	93.1 % (54 of 58)	64.3 % (36 of 56)	100.0 % (2 of 2)
Methyl	97.5 % (156 of 160)	97.5 % (78 of 80)	97.5 % (78 of 80)

1. entity 1

GSGGGAQDLS SLLPGQSSCF RPAAPNLAGA VEFSDVKTLL KEWITTISDP MEEDILQVVR YCTDLIEEKD LEKLDLVIKY MKRLMQQSVE SVWNMAFDFI LDNVQVVLQQ TYGSTLKVT

2. entity 2

SHMSHKKSFF DKKRSERISN CQDTS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
1	Rev1_CTD	[U-100% 15N]	0.7 ~ 1.0 mM
2	RIR	[U-100% 15N]	0.7 ~ 1.0 mM
3	sodium phosphate	natural abundance	25 mM
4	potassium chloride	natural abundance	100 mM
5	DTT	natural abundance	10 mM
6	EDTA	natural abundance	1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
10	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
11	sodium phosphate	natural abundance	25 mM
12	potassium chloride	natural abundance	100 mM
13	DTT	natural abundance	10 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
17	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
18	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
19	sodium phosphate	natural abundance	25 mM
20	potassium chloride	natural abundance	100 mM
21	DTT	natural abundance	10 mM
22	EDTA	natural abundance	1 mM
23	D2O	natural abundance	100 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2LSJ, Strand ID: A, B Detail

Release date

2012-06-17

Citation

Multifaceted recognition of vertebrate Rev1 by translesion polymerases ζ and κ
Wojtaszek, J., Liu, J., Wang, S., Xue, S., Walker, Y., Zhou, G.C.
J. Biol. Chem. (2012), 287, 26400-26408, PubMed 22700975 , DOI 10.1074/jbc.M112.380998 , Abstract

Related entities 1. mouse Rev1 CTD in complex with the RIR of Pol Kappa, entity 1, : 2 : 6 entities Detail

Related entities 2. mouse Rev1 CTD in complex with the RIR of Pol Kappa, entity 2, : 1 : 2 : 3 entities Detail

Interaction partners 1. mouse Rev1 CTD in complex with the RIR of Pol Kappa, entity 1, : 6 : 1 interactors Detail

More details for 7 interactors identified by 2 citations

Interaction partners 2. mouse Rev1 CTD in complex with the RIR of Pol Kappa, entity 2, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
1	Rev1_CTD	[U-100% 15N]	0.7 ~ 1.0 mM
2	RIR	[U-100% 15N]	0.7 ~ 1.0 mM
3	sodium phosphate	natural abundance	25 mM
4	potassium chloride	natural abundance	100 mM
5	DTT	natural abundance	10 mM
6	EDTA	natural abundance	1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
10	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
11	sodium phosphate	natural abundance	25 mM
12	potassium chloride	natural abundance	100 mM
13	DTT	natural abundance	10 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

3 3D HNCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
10	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
11	sodium phosphate	natural abundance	25 mM
12	potassium chloride	natural abundance	100 mM
13	DTT	natural abundance	10 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

4 3D HN(CO)CA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
10	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
11	sodium phosphate	natural abundance	25 mM
12	potassium chloride	natural abundance	100 mM
13	DTT	natural abundance	10 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

5 3D HN(CA)CB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
10	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
11	sodium phosphate	natural abundance	25 mM
12	potassium chloride	natural abundance	100 mM
13	DTT	natural abundance	10 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

6 3D HN(COCA)CB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
10	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
11	sodium phosphate	natural abundance	25 mM
12	potassium chloride	natural abundance	100 mM
13	DTT	natural abundance	10 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

7 3D HA(CA)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
10	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
11	sodium phosphate	natural abundance	25 mM
12	potassium chloride	natural abundance	100 mM
13	DTT	natural abundance	10 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

8 3D HA(CACO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
10	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
11	sodium phosphate	natural abundance	25 mM
12	potassium chloride	natural abundance	100 mM
13	DTT	natural abundance	10 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

9 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
10	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
11	sodium phosphate	natural abundance	25 mM
12	potassium chloride	natural abundance	100 mM
13	DTT	natural abundance	10 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

10 3D (HCA)CO(CA)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
10	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
11	sodium phosphate	natural abundance	25 mM
12	potassium chloride	natural abundance	100 mM
13	DTT	natural abundance	10 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

11 4D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
17	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
18	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
19	sodium phosphate	natural abundance	25 mM
20	potassium chloride	natural abundance	100 mM
21	DTT	natural abundance	10 mM
22	EDTA	natural abundance	1 mM
23	D2O	natural abundance	100 %

12 4D HCCONH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
17	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
18	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
19	sodium phosphate	natural abundance	25 mM
20	potassium chloride	natural abundance	100 mM
21	DTT	natural abundance	10 mM
22	EDTA	natural abundance	1 mM
23	D2O	natural abundance	100 %

13 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
1	Rev1_CTD	[U-100% 15N]	0.7 ~ 1.0 mM
2	RIR	[U-100% 15N]	0.7 ~ 1.0 mM
3	sodium phosphate	natural abundance	25 mM
4	potassium chloride	natural abundance	100 mM
5	DTT	natural abundance	10 mM
6	EDTA	natural abundance	1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

14 3D 1H-13C NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
17	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
18	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
19	sodium phosphate	natural abundance	25 mM
20	potassium chloride	natural abundance	100 mM
21	DTT	natural abundance	10 mM
22	EDTA	natural abundance	1 mM
23	D2O	natural abundance	100 %

15 4D 13C-HMQC-NOESY-15N-HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
9	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
10	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
11	sodium phosphate	natural abundance	25 mM
12	potassium chloride	natural abundance	100 mM
13	DTT	natural abundance	10 mM
14	EDTA	natural abundance	1 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

16 4D 13C-HMQC-NOESY-13C-HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7

#	Name	Isotope labeling	Concentration
17	Rev1_CTD	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
18	RIR	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
19	sodium phosphate	natural abundance	25 mM
20	potassium chloride	natural abundance	100 mM
21	DTT	natural abundance	10 mM
22	EDTA	natural abundance	1 mM
23	D2O	natural abundance	100 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_18433_2lsj.nef
Input source #2: Coordindates	2lsj.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
GSGGGAQDLSSLLPGQSSCFRPAAPNLAGAVEFSDVKTLLKEWITTISDPMEEDILQVVRYCTDLIEEKDLEKLDLVIKYMKRLMQQSVESVWNMAFDFI
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GSGGGAQDLSSLLPGQSSCFRPAAPNLAGAVEFSDVKTLLKEWITTISDPMEEDILQVVRYCTDLIEEKDLEKLDLVIKYMKRLMQQSVESVWNMAFDFI

-------110---------
LDNVQVVLQQTYGSTLKVT
|||||||||||||||||||
LDNVQVVLQQTYGSTLKVT

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

-------210-------220-----
SHMSHKKSFFDKKRSERISNCQDTS
|||||||||||||||||||||||||
SHMSHKKSFFDKKRSERISNCQDTS
--------10--------20-----

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	119	0	0	100.0
B	B	25	0	0	100.0

Content subtype: combined_18433_2lsj.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1388		95.0 (chain: A, length: 119), 80.0 (chain: B, length: 25)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	OK	5420 (1)	noe	92.4 (chain: A, length: 119), 64.0 (chain: B, length: 25)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_3	OK	202 (202)	.	85.7 (chain: A, length: 119), 68.0 (chain: B, length: 25)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 119, Sequence coverage: 95.0%
  - Entity_assembly_ID: B, Chain length: 25, Sequence coverage: 80.0%
- Total number of assignments
  - ¹H chemical shifts: 796
  - ¹³C chemical shifts: 460
  - ¹⁵N chemical shifts: 132
- Completeness of assignments
  - Entity_assemble_ID: A
  - Entity_assemble_ID: B
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
  - Chain_ID: B

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	716	679	94.8
¹³C chemical shifts	543	397	73.1
¹⁵N chemical shifts	131	115	87.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	241	221	91.7
¹³C chemical shifts	238	113	47.5
¹⁵N chemical shifts	115	104	90.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	475	458	96.4
¹³C chemical shifts	305	284	93.1
¹⁵N chemical shifts	16	11	68.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	81	79	97.5
¹³C chemical shifts	81	79	97.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	44	43	97.7
¹³C chemical shifts	42	27	64.3
¹⁵N chemical shifts	2	2	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	162	117	72.2
¹³C chemical shifts	113	63	55.8
¹⁵N chemical shifts	29	17	58.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	50	37	74.0
¹³C chemical shifts	50	20	40.0
¹⁵N chemical shifts	25	17	68.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	112	80	71.4
¹³C chemical shifts	63	43	68.3
¹⁵N chemical shifts	4	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	4	3	75.0
¹³C chemical shifts	4	3	75.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	14	11	78.6
¹³C chemical shifts	14	7	50.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 119, Sequence coverage: 92.4%
  - Entity_assembly_ID: B, Chain length: 25, Sequence coverage: 64.0%
- Total number of restraints: 5415
- Weight of restraints: 1.0 (5415)
- Potential type of restraints: upper-bound-parabolic (5415)
- Range of distance target values: 2.48, 7.0 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 119, Sequence coverage: 85.7%
  - Entity_assembly_ID: B, Chain length: 25, Sequence coverage: 68.0%
- Total number of restraints: 202
- Total number of restraints per polymer type: 202
- Weight of restraints: 1.0 (202)
- Potential type of restraints: square-well-parabolic (202)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A
  - Chain_ID: B

Keywords Protein/peptide complex

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Found 1 Document (0.939 seconds)

BMRB: 18433

Sample #1

Sample #2

Sample #3

Related entities 1. mouse Rev1 CTD in complex with the RIR of Pol Kappa, entity 1, : 2 : 6 entities Detail

Related entities 2. mouse Rev1 CTD in complex with the RIR of Pol Kappa, entity 2, : 1 : 2 : 3 entities Detail

Interaction partners 1. mouse Rev1 CTD in complex with the RIR of Pol Kappa, entity 1, : 6 : 1 interactors Detail

Interaction partners 2. mouse Rev1 CTD in complex with the RIR of Pol Kappa, entity 2, : 1 interactors Detail

Experiments performed 16 experiments Detail

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NMR combined restraints 4 contents Detail